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Title: | Localization of NO synthase in rat brain by [3H]L-NG-nitro-arginine autoradiography. | Austin Authors: | Burazin, T C;Gundlach, Andrew L | Affiliation: | University of Melbourne, Department of Medicine, Austin and Repatriation Medical Centre, Heidelberg, Victoria, Australia | Issue Date: | 2-Oct-1995 | Publication information: | Neuroreport; 6(14): 1842-4 | Abstract: | Nitric oxide (NO), a free radical gas that acts as a neural messenger, is produced from L-arginine by the enzyme nitric oxide synthase (NOS). The present study describes the autoradiographic localization throughout rat brain of the binding of [3H]L-NG-nitro-arginine ([3H]L-NOARG), a reversible NOS inhibitor, and confirms the heterogeneous distribution of the NOS enzyme. [3H]L-NOARG binding was enriched in discrete neuronal populations and the distribution observed was identical to that previously described for NOS using immunohistochemistry, in situ hybridization and NADPH-diaphorase staining. [3H]L-NOARG autoradiography should be a useful method for the further characterization and quantification of NOS in brain and other tissues. | Gov't Doc #: | 8547580 | URI: | http://ahro.austin.org.au/austinjspui/handle/1/13405 | Journal: | Neuroreport | URL: | https://pubmed.ncbi.nlm.nih.gov/8547580 | Type: | Journal Article | Subjects: | Animals Arginine.analogs & derivatives.metabolism Autoradiography Enzyme Inhibitors.metabolism Male Nitric Oxide Synthase.analysis.antagonists & inhibitors Nitroarginine Rats Rats, Sprague-Dawley Rats, Wistar Tritium.diagnostic use |
Appears in Collections: | Journal articles |
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