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Title: Localization of NO synthase in rat brain by [3H]L-NG-nitro-arginine autoradiography.
Austin Authors: Burazin, T C;Gundlach, Andrew L
Affiliation: University of Melbourne, Department of Medicine, Austin and Repatriation Medical Centre, Heidelberg, Victoria, Australia
Issue Date: 2-Oct-1995
Publication information: Neuroreport; 6(14): 1842-4
Abstract: Nitric oxide (NO), a free radical gas that acts as a neural messenger, is produced from L-arginine by the enzyme nitric oxide synthase (NOS). The present study describes the autoradiographic localization throughout rat brain of the binding of [3H]L-NG-nitro-arginine ([3H]L-NOARG), a reversible NOS inhibitor, and confirms the heterogeneous distribution of the NOS enzyme. [3H]L-NOARG binding was enriched in discrete neuronal populations and the distribution observed was identical to that previously described for NOS using immunohistochemistry, in situ hybridization and NADPH-diaphorase staining. [3H]L-NOARG autoradiography should be a useful method for the further characterization and quantification of NOS in brain and other tissues.
Gov't Doc #: 8547580
Journal: Neuroreport
Type: Journal Article
Subjects: Animals
Arginine.analogs & derivatives.metabolism
Enzyme Inhibitors.metabolism
Nitric Oxide Synthase.analysis.antagonists & inhibitors
Rats, Sprague-Dawley
Rats, Wistar
Tritium.diagnostic use
Appears in Collections:Journal articles

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