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Title: Secretory and biosynthetic responses of gastrin and somatostatin to acute changes in gastric acidity.
Austin Authors: Kapuscinski, M;Shulkes, Arthur
Affiliation: University of Melbourne Department of Surgery, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 8-Jul-1995
Publication information: Journal of Gastroenterology and Hepatology; 10(4): 405-12
Abstract: The activity of gastric parietal cells in terms of hydrochloric acid (HCl) secretion is regulated by the interaction of stimulatory substances (e.g. gastrin) and inhibitors (e.g. somatostatin) acting in an endocrine and paracrine mode, as well as luminal factors. In the present study the following parameters were measured: the synthesis (mRNA), storage (tissue peptide concentration) and secretion (plasma peptide concentration) of somatostatin and gastrin following short-term treatment of rats with pentagastrin (acid stimulant), secretin, omeprazole (reduces gastric acidity by inactivating gastric H/K ATPase) and the somatostatin analogue octreotide (reduces gastric acidity by inhibiting both the parietal cell and gastrin). The mRNA coding for H/K ATPase and carbonic anhydrase II (CA II), the two enzymes responsible for the generation of hydrogen ions from the parietal cell, were also quantitated. In response to octreotide, somatostatin peptide and mRNA levels in the fundus rose to 180 +/- 16% (P < 0.001) and 1073 +/- 356% (P < 0.05) of control, respectively. In contrast, octreotide caused a decrease in antral somatostatin peptide and its mRNA did not change significantly. No significant changes in synthesis, secretion or storage of gastrin were observed except for omeprazole induced hypergastrinaemia (580 +/- 76%, P < 0.001). H/K ATPase and CA II mRNA were largely unaffected except for an increase in CA II mRNA following octreotide and a decrease in H/K ATPase mRNA after pentagastrin. These data support the concept of the differential control of antral and fundic somatostatin synthesis and provide evidence for a regulatory loop by which somatostatin can influence its own synthesis.(ABSTRACT TRUNCATED AT 250 WORDS)
Gov't Doc #: 8527706
Journal: Journal of Gastroenterology and Hepatology
Type: Journal Article
Subjects: Adenosine Triphosphatases.drug effects.genetics.metabolism
Carbonic Anhydrases.drug effects.genetics.metabolism
Enzyme Inhibitors.pharmacology
Gastric Acid.metabolism
Gastric Mucosa.drug effects.secretion
Gastrins.biosynthesis.drug effects.genetics
Gene Expression Regulation
Hydrogen-Ion Concentration
RNA, Messenger.metabolism
Rats, Sprague-Dawley
Somatostatin.biosynthesis.drug effects.genetics
Appears in Collections:Journal articles

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