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DC Field | Value | Language |
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dc.contributor.author | Jennings, J G | en |
dc.contributor.author | Chang, L | en |
dc.contributor.author | Savige, Judy A | en |
dc.date.accessioned | 2015-05-16T03:08:42Z | |
dc.date.available | 2015-05-16T03:08:42Z | |
dc.date.issued | 1994-02-01 | en |
dc.identifier.citation | Clinical and Experimental Immunology; 95(2): 251-6 | en |
dc.identifier.govdoc | 8306500 | en |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/13316 | en |
dc.description.abstract | Anti-proteinase 3 antibodies are a subgroup of anti-neutrophil cytoplasmic antibodies (ANCA), and we have established an ELISA for their detection using high performance liquid chromatography (HPLC)-purified protein. This assay is sensitive and specific: inhibition studies have shown that despite the homology between proteinase 3 and elastase there is no cross-reactivity between the corresponding antibodies for their targets. Anti-proteinase 3 antibodies were associated most often with cytoplasmic fluorescence (17/22, 77%), but occasionally with a perinuclear (3/22, 14%) or atypical pattern (1/2). These antibodies were found in 23 out of 76 sera (30%) that were positive in an ELISA based on a crude neutrophil cytoplasmic extract, and they were associated with both 29 and 55 kD bands on Western blots. Anti-proteinase 3 antibodies were found in most individuals with active Wegener's granulomatosis (10/13, 77%), but less often in individuals with microscopic polyarteritis (2/10, 20%) or segmental necrotizing glomerulonephritis (3/6, 50%). However, anti-proteinase 3 antibodies were not detected in any of 32 sera from individuals with rheumatoid arthritis or systemic lupus erythematosus (SLE). Occasionally anti-proteinase 3 antibodies were associated with anti-glomerular basement membrane antibodies (1/11, 9%) or with anti-myeloperoxidase antibodies (1/11, 9%). IgM anti-proteinase 3 antibodies were uncommon (2/22 sera, 9%), and no IgA antibodies were demonstrated in any of 22 sera from patients with active systemic vasculitis. Significantly more individuals presented with anti-proteinase 3 antibodies in April-May-June, suggesting that an infective agent prevalent in Autumn might have a causative role in the associated diseases. Anti-proteinase 3 antibodies are the most common target antigen associated with Wegener's granulomatosis and cytoplasmic fluorescence. | en |
dc.language.iso | en | en |
dc.subject.other | Antibodies, Antineutrophil Cytoplasmic | en |
dc.subject.other | Autoantibodies.analysis.immunology | en |
dc.subject.other | Autoantigens.immunology | en |
dc.subject.other | Enzyme-Linked Immunosorbent Assay | en |
dc.subject.other | Granulomatosis with Polyangiitis.immunology | en |
dc.subject.other | Humans | en |
dc.subject.other | Immunoglobulin Isotypes.analysis | en |
dc.subject.other | Myeloblastin | en |
dc.subject.other | Seasons | en |
dc.subject.other | Serine Endopeptidases.immunology | en |
dc.title | Anti-proteinase 3 antibodies, their characterization and disease associations. | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | Clinical and experimental immunology | en |
dc.identifier.affiliation | Department of Haematology, Heidelberg Repatriation Hospital, Victoria, Australia | en |
dc.description.pages | 251-6 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/8306500 | en |
dc.type.austin | Journal Article | en |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.openairetype | Journal Article | - |
Appears in Collections: | Journal articles |
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