Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13316
Full metadata record
DC FieldValueLanguage
dc.contributor.authorJennings, J Gen
dc.contributor.authorChang, Len
dc.contributor.authorSavige, Judy Aen
dc.date.accessioned2015-05-16T03:08:42Z
dc.date.available2015-05-16T03:08:42Z
dc.date.issued1994-02-01en
dc.identifier.citationClinical and Experimental Immunology; 95(2): 251-6en
dc.identifier.govdoc8306500en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/13316en
dc.description.abstractAnti-proteinase 3 antibodies are a subgroup of anti-neutrophil cytoplasmic antibodies (ANCA), and we have established an ELISA for their detection using high performance liquid chromatography (HPLC)-purified protein. This assay is sensitive and specific: inhibition studies have shown that despite the homology between proteinase 3 and elastase there is no cross-reactivity between the corresponding antibodies for their targets. Anti-proteinase 3 antibodies were associated most often with cytoplasmic fluorescence (17/22, 77%), but occasionally with a perinuclear (3/22, 14%) or atypical pattern (1/2). These antibodies were found in 23 out of 76 sera (30%) that were positive in an ELISA based on a crude neutrophil cytoplasmic extract, and they were associated with both 29 and 55 kD bands on Western blots. Anti-proteinase 3 antibodies were found in most individuals with active Wegener's granulomatosis (10/13, 77%), but less often in individuals with microscopic polyarteritis (2/10, 20%) or segmental necrotizing glomerulonephritis (3/6, 50%). However, anti-proteinase 3 antibodies were not detected in any of 32 sera from individuals with rheumatoid arthritis or systemic lupus erythematosus (SLE). Occasionally anti-proteinase 3 antibodies were associated with anti-glomerular basement membrane antibodies (1/11, 9%) or with anti-myeloperoxidase antibodies (1/11, 9%). IgM anti-proteinase 3 antibodies were uncommon (2/22 sera, 9%), and no IgA antibodies were demonstrated in any of 22 sera from patients with active systemic vasculitis. Significantly more individuals presented with anti-proteinase 3 antibodies in April-May-June, suggesting that an infective agent prevalent in Autumn might have a causative role in the associated diseases. Anti-proteinase 3 antibodies are the most common target antigen associated with Wegener's granulomatosis and cytoplasmic fluorescence.en
dc.language.isoenen
dc.subject.otherAntibodies, Antineutrophil Cytoplasmicen
dc.subject.otherAutoantibodies.analysis.immunologyen
dc.subject.otherAutoantigens.immunologyen
dc.subject.otherEnzyme-Linked Immunosorbent Assayen
dc.subject.otherGranulomatosis with Polyangiitis.immunologyen
dc.subject.otherHumansen
dc.subject.otherImmunoglobulin Isotypes.analysisen
dc.subject.otherMyeloblastinen
dc.subject.otherSeasonsen
dc.subject.otherSerine Endopeptidases.immunologyen
dc.titleAnti-proteinase 3 antibodies, their characterization and disease associations.en
dc.typeJournal Articleen
dc.identifier.journaltitleClinical and experimental immunologyen
dc.identifier.affiliationDepartment of Haematology, Heidelberg Repatriation Hospital, Victoria, Australiaen
dc.description.pages251-6en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/8306500en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

4
checked on Mar 28, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.