Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13312
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dc.contributor.authorChang, Len
dc.contributor.authorSavige, Judy Aen
dc.date.accessioned2015-05-16T03:08:26Z
dc.date.available2015-05-16T03:08:26Z
dc.date.issued1993-05-16en
dc.identifier.citationAdvances in Experimental Medicine and Biology; 336(): 97-100en
dc.identifier.govdoc8296682en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/13312en
dc.description.abstractMany autoantibodies that have enzymes as their targets bind to the catalytic sites and inhibit enzymatic activity. We have established functional assays for the targets of anti-neutrophil cytoplasm antibodies (ANCA) in order to determine whether these antibodies are directed against the corresponding catalytic sites. Anti-proteinase 3 activity was measured by the cleavage of alpha-naphthyl acetate; myeloperoxidase activity by peroxidation of monochlorodimedon or guaiacol using hydrogen peroxide; and elastase activity by a fluorimetric assay of the hydrolytic product of N-succ (ala)3 amido-methyl coumarin. The addition of immunoglobulin from patients with ANCA to these assays did not result in inhibition of functional activity of the corresponding enzyme when compared with normal immunoglobulin. Furthermore the removal of specific immunoglobulin by solid phase adsorption to the corresponding antigen did not result in an increase in enzymatic activity compared with the starting material. However preliminary results suggest that anti-neutrophil proteinase 3 binding may be inhibited in a solid-phase ELISA by preincubation with a peptide corresponding to the catalytic site.en
dc.language.isoenen
dc.subject.otherAntibodies, Antineutrophil Cytoplasmicen
dc.subject.otherAutoantibodies.pharmacologyen
dc.subject.otherBinding Sitesen
dc.subject.otherHumansen
dc.subject.otherImmunoglobulin G.pharmacologyen
dc.subject.otherLysosomes.enzymologyen
dc.subject.otherMyeloblastinen
dc.subject.otherNeutrophils.enzymologyen
dc.subject.otherPancreatic Elastase.antagonists & inhibitorsen
dc.subject.otherPeroxidase.antagonists & inhibitorsen
dc.subject.otherProtease Inhibitors.pharmacologyen
dc.subject.otherSerine Endopeptidases.metabolismen
dc.titleStudies to demonstrate inhibition of functional activity of neutrophil lysosomal enzymes with ANCA.en
dc.typeJournal Articleen
dc.identifier.journaltitleAdvances in Experimental Medicine and Biologyen
dc.identifier.affiliationDepartment of Haematology, Heidelberg Repatriation Hospital Victoria, Australiaen
dc.description.pages97-100en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/8296682en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
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