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Title: Electrophysiological and autoradiographical evidence for cholecystokinin A receptors on rat isolated nodose ganglia.
Austin Authors: Widdop, Robert E;Krstew, E;Mercer, L D;Carlberg, M;Beart, P M;Jarrott, B
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 2-Jan-1994
Publication information: Journal of the Autonomic Nervous System; 46(1-2): 65-73
Abstract: The sulphated octapeptide, cholecystokinin (CCK-8S), is believed to be a neurotransmitter of vagal sensory neurones, and here the presence of functional receptors for CCK-8S in the rat vagus nerve has been investigated by electrophysiological and autoradiographic techniques. CCK-8S caused concentration-dependent depolarizations when superfused over the rat isolated nodose ganglion at 37 degrees C as measured by a silicone grease gap technique. Concentration-response curves to CCK-8S were shifted to the right by low concentrations of the CCKA receptor antagonist, Devazepide, but not by the CCKB receptor antagonist, L-365,260, data which indicate that receptors were of the CCKA subtype. Consistent with this notion, the CCKB agonist, unsulphated CCK-8, was without effect until high concentrations (> 1 microM) were used. A synthetic analogue of CCK-8S, D-Tyr25(Nle28,31)-CCK 25-33S, which has been reported to be more stable and peptidase-resistant than CCK-8S, was equipotent with CCK-8S in depolarizing the nodose ganglion. When D-Tyr25(Nle28,31)-CCK 25-33S was labelled with 125I, it bound to tissue sections of nodose ganglion. By light microscopic autoradiography, silver grains were found to be highly localized over cell bodies of vagal sensory neurones. An excess of CCK-8S inhibited binding as did Devazepide, but not L-365,260, confirming that binding sites were CCKA subtype receptors. These results indicate the existence of functional CCKA receptors in the nodose ganglion and strengthen the case for the involvement of vagal sensory neurones in gastric emptying and satiety.
Gov't Doc #: 8120343
Journal: Journal of the autonomic nervous system
Type: Journal Article
Subjects: Animals
Cholecystokinin.antagonists & inhibitors
In Vitro Techniques
Iodine Radioisotopes.diagnostic use
Nodose Ganglion.cytology.metabolism
Phenylurea Compounds
Rats, Sprague-Dawley
Receptors, Cholecystokinin.antagonists & inhibitors.metabolism.physiology
Silver Staining
Vagus Nerve.cytology.metabolism
Appears in Collections:Journal articles

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