Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13252
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dc.contributor.authorWiddop, Robert Een
dc.contributor.authorKrstew, Een
dc.contributor.authorMercer, L Den
dc.contributor.authorCarlberg, Men
dc.contributor.authorBeart, P Men
dc.contributor.authorJarrott, Ben
dc.date.accessioned2015-05-16T03:04:19Z
dc.date.available2015-05-16T03:04:19Z
dc.date.issued1994-01-02en
dc.identifier.citationJournal of the Autonomic Nervous System; 46(1-2): 65-73en
dc.identifier.govdoc8120343en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/13252en
dc.description.abstractThe sulphated octapeptide, cholecystokinin (CCK-8S), is believed to be a neurotransmitter of vagal sensory neurones, and here the presence of functional receptors for CCK-8S in the rat vagus nerve has been investigated by electrophysiological and autoradiographic techniques. CCK-8S caused concentration-dependent depolarizations when superfused over the rat isolated nodose ganglion at 37 degrees C as measured by a silicone grease gap technique. Concentration-response curves to CCK-8S were shifted to the right by low concentrations of the CCKA receptor antagonist, Devazepide, but not by the CCKB receptor antagonist, L-365,260, data which indicate that receptors were of the CCKA subtype. Consistent with this notion, the CCKB agonist, unsulphated CCK-8, was without effect until high concentrations (> 1 microM) were used. A synthetic analogue of CCK-8S, D-Tyr25(Nle28,31)-CCK 25-33S, which has been reported to be more stable and peptidase-resistant than CCK-8S, was equipotent with CCK-8S in depolarizing the nodose ganglion. When D-Tyr25(Nle28,31)-CCK 25-33S was labelled with 125I, it bound to tissue sections of nodose ganglion. By light microscopic autoradiography, silver grains were found to be highly localized over cell bodies of vagal sensory neurones. An excess of CCK-8S inhibited binding as did Devazepide, but not L-365,260, confirming that binding sites were CCKA subtype receptors. These results indicate the existence of functional CCKA receptors in the nodose ganglion and strengthen the case for the involvement of vagal sensory neurones in gastric emptying and satiety.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAutoradiographyen
dc.subject.otherBenzodiazepinones.pharmacologyen
dc.subject.otherCholecystokinin.antagonists & inhibitorsen
dc.subject.otherDevazepideen
dc.subject.otherElectrophysiologyen
dc.subject.otherIn Vitro Techniquesen
dc.subject.otherIodine Radioisotopes.diagnostic useen
dc.subject.otherMaleen
dc.subject.otherNodose Ganglion.cytology.metabolismen
dc.subject.otherPhenylurea Compoundsen
dc.subject.otherRatsen
dc.subject.otherRats, Sprague-Dawleyen
dc.subject.otherReceptors, Cholecystokinin.antagonists & inhibitors.metabolism.physiologyen
dc.subject.otherSerotonin.pharmacologyen
dc.subject.otherSilver Stainingen
dc.subject.otherSincalide.pharmacologyen
dc.subject.otherVagus Nerve.cytology.metabolismen
dc.titleElectrophysiological and autoradiographical evidence for cholecystokinin A receptors on rat isolated nodose ganglia.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of the autonomic nervous systemen
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.description.pages65-73en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/8120343en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
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