Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13245
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWall, D Men
dc.contributor.authorSparrow, Ren
dc.contributor.authorHu, X Fen
dc.contributor.authorNadalin, Gen
dc.contributor.authorZalcberg, John Ren
dc.contributor.authorMarschner, I Cen
dc.contributor.authorVan der Weyden, Men
dc.contributor.authorParkin, John Desmonden
dc.date.accessioned2015-05-16T03:03:51Z
dc.date.available2015-05-16T03:03:51Z
dc.date.issued1993-05-16en
dc.identifier.citationEuropean Journal of Cancer (oxford, England : 1990); 29A(7): 1024-7en
dc.identifier.govdoc8098947en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/13245en
dc.description.abstractA rapid and simple functional assay for P-glycoprotein (Pgp) using flow cytometry to measure the accumulation of the flurophore fluo-3 has been applied to samples from patients with B-cell chronic lymphocytic leukaemia (B-CLL). Peripheral blood lymphocytes from 37 patients with B-CLL were studied for Pgp. Pgp expression, using MRK-16, a monoclonal antibody recognising an external surface epitope of Pgp, was detected in 92% of patients with B-CLL. The functional assays for Pgp expression were positive in 78 and 59% of patients using the fluo-3 and doxorubicin (dox) assays, respectively. When compared with the MRK-16 assay, the fluo-3 assay had a sensitivity of 82% compared to a sensitivity of 56% for the dox assay (P = 0.004). The specificity of the fluo-3 and dox assays could not be evaluated because of the low number of MRK-16 negative CLL cells.en
dc.language.isoenen
dc.subject.otherAgeden
dc.subject.otherAged, 80 and overen
dc.subject.otherAniline Compounds.metabolismen
dc.subject.otherCarrier Proteins.analysisen
dc.subject.otherDoxorubicin.metabolismen
dc.subject.otherDrug Resistanceen
dc.subject.otherFlow Cytometryen
dc.subject.otherFluorescent Dyes.metabolismen
dc.subject.otherHumansen
dc.subject.otherLeukemia, Lymphocytic, Chronic, B-Cell.blood.drug therapy.metabolismen
dc.subject.otherLymphocytes.metabolismen
dc.subject.otherMembrane Glycoproteins.analysisen
dc.subject.otherMiddle Ageden
dc.subject.otherNeoplasm Proteins.blooden
dc.subject.otherP-Glycoproteinen
dc.subject.otherSensitivity and Specificityen
dc.subject.otherXanthenes.metabolismen
dc.titleClinical application of a rapid, functional assay for multidrug resistance based on accumulation of the fluorescent dye, fluo-3.en
dc.typeJournal Articleen
dc.identifier.journaltitleEuropean journal of cancer (Oxford, England : 1990)en
dc.identifier.affiliationDepartment of Haematology, Heidelberg Repatriation Hospital.en
dc.description.pages1024-7en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/8098947en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
Appears in Collections:Journal articles
Show simple item record

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.