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Title: | Clinical application of a rapid, functional assay for multidrug resistance based on accumulation of the fluorescent dye, fluo-3. | Austin Authors: | Wall, D M;Sparrow, R;Hu, X F;Nadalin, G;Zalcberg, John R;Marschner, I C;Van der Weyden, M;Parkin, John Desmond | Affiliation: | Department of Haematology, Heidelberg Repatriation Hospital. | Issue Date: | 16-May-1993 | Publication information: | European Journal of Cancer (oxford, England : 1990); 29A(7): 1024-7 | Abstract: | A rapid and simple functional assay for P-glycoprotein (Pgp) using flow cytometry to measure the accumulation of the flurophore fluo-3 has been applied to samples from patients with B-cell chronic lymphocytic leukaemia (B-CLL). Peripheral blood lymphocytes from 37 patients with B-CLL were studied for Pgp. Pgp expression, using MRK-16, a monoclonal antibody recognising an external surface epitope of Pgp, was detected in 92% of patients with B-CLL. The functional assays for Pgp expression were positive in 78 and 59% of patients using the fluo-3 and doxorubicin (dox) assays, respectively. When compared with the MRK-16 assay, the fluo-3 assay had a sensitivity of 82% compared to a sensitivity of 56% for the dox assay (P = 0.004). The specificity of the fluo-3 and dox assays could not be evaluated because of the low number of MRK-16 negative CLL cells. | Gov't Doc #: | 8098947 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/13245 | Journal: | European journal of cancer (Oxford, England : 1990) | URL: | https://pubmed.ncbi.nlm.nih.gov/8098947 | Type: | Journal Article | Subjects: | Aged Aged, 80 and over Aniline Compounds.metabolism Carrier Proteins.analysis Doxorubicin.metabolism Drug Resistance Flow Cytometry Fluorescent Dyes.metabolism Humans Leukemia, Lymphocytic, Chronic, B-Cell.blood.drug therapy.metabolism Lymphocytes.metabolism Membrane Glycoproteins.analysis Middle Aged Neoplasm Proteins.blood P-Glycoprotein Sensitivity and Specificity Xanthenes.metabolism |
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