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Title: Clinical application of a rapid, functional assay for multidrug resistance based on accumulation of the fluorescent dye, fluo-3.
Austin Authors: Wall, D M;Sparrow, R;Hu, X F;Nadalin, G;Zalcberg, John R;Marschner, I C;Van der Weyden, M;Parkin, John Desmond
Affiliation: Department of Haematology, Heidelberg Repatriation Hospital.
Issue Date: 16-May-1993
Publication information: European Journal of Cancer (oxford, England : 1990); 29A(7): 1024-7
Abstract: A rapid and simple functional assay for P-glycoprotein (Pgp) using flow cytometry to measure the accumulation of the flurophore fluo-3 has been applied to samples from patients with B-cell chronic lymphocytic leukaemia (B-CLL). Peripheral blood lymphocytes from 37 patients with B-CLL were studied for Pgp. Pgp expression, using MRK-16, a monoclonal antibody recognising an external surface epitope of Pgp, was detected in 92% of patients with B-CLL. The functional assays for Pgp expression were positive in 78 and 59% of patients using the fluo-3 and doxorubicin (dox) assays, respectively. When compared with the MRK-16 assay, the fluo-3 assay had a sensitivity of 82% compared to a sensitivity of 56% for the dox assay (P = 0.004). The specificity of the fluo-3 and dox assays could not be evaluated because of the low number of MRK-16 negative CLL cells.
Gov't Doc #: 8098947
Journal: European journal of cancer (Oxford, England : 1990)
Type: Journal Article
Subjects: Aged
Aged, 80 and over
Aniline Compounds.metabolism
Carrier Proteins.analysis
Drug Resistance
Flow Cytometry
Fluorescent Dyes.metabolism
Leukemia, Lymphocytic, Chronic, B-Cell.blood.drug therapy.metabolism
Membrane Glycoproteins.analysis
Middle Aged
Neoplasm Proteins.blood
Sensitivity and Specificity
Appears in Collections:Journal articles

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