Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13192
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dc.contributor.authorDean, Rachael Gen
dc.contributor.authorZhuo, Jen
dc.contributor.authorAlcorn, Den
dc.contributor.authorCasley, David Jen
dc.contributor.authorMendelsohn, Frederick AOen
dc.date.accessioned2015-05-16T02:59:08Z
dc.date.available2015-05-16T02:59:08Z
dc.date.issued1994-11-01en
dc.identifier.citationThe American Journal of Physiology; 267(5 Pt 2): F845-52en
dc.identifier.govdoc7977789en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/13192en
dc.description.abstractEndothelin-1 (ET-1) receptors have previously been demonstrated in the rat kidney by in vitro autoradiography and in cultured renal cell lines by radioreceptor assay, but the precise cellular localization of these receptors under in vivo conditions remains to be determined. We performed electron microscopic autoradiography on rat kidney following intravenous administration of 125I-labeled ET-1. In vivo autoradiographs revealed binding patterns identical to those previously demonstrated following in vitro labeling. Light microscopic autoradiography showed that silver grains occurred exclusively overlaying glomeruli and peritubular capillaries in the cortex, inner stripe of the outer medulla, and the inner medulla. At the electron microscopic level, ET-1 binding was specifically localized to the fenestrated endothelium of glomerular and peritubular capillaries, and to a lesser extent to the vasa recta. No significant grains were seen on mesangial or visceral epithelial cells; nor were any seen on the cells of proximal tubule, the thick and thin limbs of the loop of Henle, the medullary collecting ducts, and renal interstitial cells. These results indicate that the endothelial cells of glomerular and peritubular capillaries are the primary target for the circulating ET-1 in the rat kidney and suggest an autocrine and/or paracrine function of locally synthesized ET-1 in vivo in both physiological and pathophysiological states.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAutoradiographyen
dc.subject.otherCapillaries.metabolismen
dc.subject.otherEndothelins.metabolismen
dc.subject.otherEndothelium, Vascular.metabolismen
dc.subject.otherGlomerular Mesangium.metabolismen
dc.subject.otherIodine Radioisotopesen
dc.subject.otherKidney.cytology.metabolism.ultrastructureen
dc.subject.otherKidney Cortex.metabolismen
dc.subject.otherKidney Glomerulus.metabolismen
dc.subject.otherKidney Tubules.metabolismen
dc.subject.otherLoop of Henle.metabolismen
dc.subject.otherMaleen
dc.subject.otherMicroscopy, Electronen
dc.subject.otherRadioligand Assayen
dc.subject.otherRatsen
dc.subject.otherRats, Sprague-Dawleyen
dc.subject.otherReceptors, Endothelin.metabolismen
dc.subject.otherRenal Circulationen
dc.titleCellular distribution of 125I-endothelin-1 binding in rat kidney following in vivo labeling.en
dc.typeJournal Articleen
dc.identifier.journaltitleAmerican Journal of Physiologyen
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Austin Hospital, Heidelberg.en
dc.description.pagesF845-52en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/7977789en
dc.type.austinJournal Articleen
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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