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Title: Production of anti-breast cancer monoclonal antibodies using a glutathione-S-transferase-MUC1 bacterial fusion protein.
Austin Authors: Apostolopoulos, V;Xing, Pei Xiang;Trapani, Joseph A;McKenzie, Ian F C
Affiliation: Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 1-Apr-1993
Publication information: British Journal of Cancer; 67(4): 713-20
Abstract: Two murine Mabs VA1(IgG1) and VA2(IgG1) were produced against a bacterial fusion protein comprising glutathione S-transferase and five tandem repeats of the MUC1 protein. Using the immunoperoxidase staining technique, VA1 detected 46/53 and VA2 detected 48/53 breast cancers and both also reacted with a range of other human epithelial carcinomas. In addition VA1 gave weak reactions with normal breast tissues whereas VA2 was non-reactive and could be a relatively tumour specific antibody for breast cancer. The antibodies were also tested by ELISA-VA1 reacted weakly with glycosylated HMFG but strongly with deglycosylated HMFG, whereas VA2 reacted strongly with both forms of HMFG. The reactivities of the two Mabs with synthetic peptides of the MUC1 tandem repeat were used to map the epitopes recognised by VA1 (amino acids RPAPGS) and VA2 (amino acids DTRPA). The use of fusion proteins provides another means of immunisation to produce anti-tumour antibodies.
Gov't Doc #: 7682431
Type: Journal Article
Subjects: Amino Acid Sequence
Antibodies, Monoclonal.biosynthesis
Antibodies, Neoplasm.biosynthesis
Antibody Specificity
Bacterial Proteins.genetics.immunology
Breast Neoplasms.immunology
Glutathione Transferase.genetics.immunology
Membrane Glycoproteins.genetics.immunology
Mice, Inbred BALB C
Mice, Inbred CBA
Molecular Sequence Data
Neoplasm Proteins.genetics.immunology
Recombinant Fusion Proteins.immunology
Appears in Collections:Journal articles

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