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Title: Extracellular ATP increases NH4+ permeability in human lymphocytes by opening a P2Z purinoceptor operated ion channel.
Austin Authors: Chen, J R;Jamieson, Gary P;Wiley, J S
Affiliation: Department of Haematology, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 15-Aug-1994
Publication information: Biochemical and Biophysical Research Communications; 202(3): 1511-6
Abstract: The permeability of lymphocytes to NH4+ was examined by measuring intracellular pH using the fluorescent pH-sensitive dye BCECF. Addition of 20 mM NH4Cl produced a rapid phase of alkalinization. This was followed by a slow return to resting pHi due to NH4+ influx. The rate of NH4+ was increased many fold by extracellular ATP and the increment showed features consistent with NH4+ being a permeant for the P2Z purinoceptor operated ion channel. Cytosolic pH measurements showed monomethylammonium+ and dimethylammonium+ were also permeants, but trimethylammonium+ (69 Dalton) was excluded by this channel. Since our previous data showed ethidium+ (314 Dalton) is a permeant it appears that molecular conformation rather than molecular weight determines entry of cationic solutes through the channel.
Gov't Doc #: 7520239
DOI: 10.1006/bbrc.1994.2102
Type: Journal Article
Subjects: Adenosine Triphosphate.physiology
Amiloride.analogs & derivatives.pharmacology
Biological Transport
Cell Membrane Permeability
Fluorescent Dyes
Hydrogen-Ion Concentration
Ion Channels.physiology
Quaternary Ammonium Compounds.metabolism
Receptors, Purinergic.physiology
Appears in Collections:Journal articles

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