Please use this identifier to cite or link to this item:
|Title:||Extracellular ATP increases NH4+ permeability in human lymphocytes by opening a P2Z purinoceptor operated ion channel.||Austin Authors:||Chen, J R;Jamieson, Gary P;Wiley, J S||Affiliation:||Department of Haematology, Austin Hospital, Heidelberg, Victoria, Australia||Issue Date:||15-Aug-1994||Publication information:||Biochemical and Biophysical Research Communications; 202(3): 1511-6||Abstract:||The permeability of lymphocytes to NH4+ was examined by measuring intracellular pH using the fluorescent pH-sensitive dye BCECF. Addition of 20 mM NH4Cl produced a rapid phase of alkalinization. This was followed by a slow return to resting pHi due to NH4+ influx. The rate of NH4+ was increased many fold by extracellular ATP and the increment showed features consistent with NH4+ being a permeant for the P2Z purinoceptor operated ion channel. Cytosolic pH measurements showed monomethylammonium+ and dimethylammonium+ were also permeants, but trimethylammonium+ (69 Dalton) was excluded by this channel. Since our previous data showed ethidium+ (314 Dalton) is a permeant it appears that molecular conformation rather than molecular weight determines entry of cationic solutes through the channel.||Gov't Doc #:||7520239||URI:||http://ahro.austin.org.au/austinjspui/handle/1/13024||DOI:||10.1006/bbrc.1994.2102||URL:||https://pubmed.ncbi.nlm.nih.gov/7520239||Type:||Journal Article||Subjects:||Adenosine Triphosphate.physiology
Amiloride.analogs & derivatives.pharmacology
Cell Membrane Permeability
Quaternary Ammonium Compounds.metabolism
|Appears in Collections:||Journal articles|
Show full item record
Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.