Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/13020
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dc.contributor.authorGürtler, Ven
dc.date.accessioned2015-05-16T02:47:37Z
dc.date.available2015-05-16T02:47:37Z
dc.date.issued1993-12-01en
dc.identifier.citationJournal of General Microbiology; 139(12): 3089-97en
dc.identifier.govdoc7510324en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/13020en
dc.description.abstractTo develop a rapid and accurate method of typing large numbers of clinical isolates of Clostridium difficile, four regions of the rRNA operon [A, 15-1407 and B, 907-1407 (16S-16S); C, 1392-507 and D, 907-507 (16S-23S)] were enzymically amplified from 24 strains. When region A was hybridized to HindIII-digested genomic DNA isolated from C. difficile strains, all of the variable length restriction fragments hybridized. When region B was hybridized to HindIII-digested genomic DNA isolated from C. difficile strains, a set of variable length restriction fragments (Group II) hybridized predominantly. When region C was separated by agarose gel electrophoresis, a series of products ranging in size from approximately 800-1300 bp was obtained. When regions C and D were digested with HindIII, a constant region of 430 bp was found in both products and in all strains. From the above experiments it was concluded that the variable length Group II restriction fragments and the variable length region C amplification products were due to variable length 16S-23S spacer regions between alleles of the one strain. When region C amplification products were separated by denaturing PAGE, 16 variable length rRNA alleles (rrnA-P) were demonstrated from 24 C. difficile strains ranging in size from 852-1210 bp. After analysis with maximum parsimony, the 24 strains were divided into 14 ribotypes.(ABSTRACT TRUNCATED AT 250 WORDS)en
dc.language.isoenen
dc.subject.otherBacterial Typing Techniquesen
dc.subject.otherBase Sequenceen
dc.subject.otherClostridium difficile.classification.genetics.isolation & purificationen
dc.subject.otherDNA Primers.geneticsen
dc.subject.otherDNA, Bacterial.geneticsen
dc.subject.otherDNA, Ribosomal.geneticsen
dc.subject.otherGenetic Variationen
dc.subject.otherHumansen
dc.subject.otherMolecular Sequence Dataen
dc.subject.otherOperonen
dc.subject.otherPolymerase Chain Reaction.methodsen
dc.subject.otherRNA, Bacterial.geneticsen
dc.subject.otherRNA, Ribosomal, 16S.geneticsen
dc.subject.otherRNA, Ribosomal, 23S.geneticsen
dc.titleTyping of Clostridium difficile strains by PCR-amplification of variable length 16S-23S rDNA spacer regions.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of general microbiologyen
dc.identifier.affiliationDepartment of Microbiology, Heidelberg Repatriation Hospital, Australiaen
dc.description.pages3089-97en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/7510324en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
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