Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/12968
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dc.contributor.authorElz, J Sen
dc.contributor.authorNayler, W Gen
dc.date.accessioned2015-05-16T02:44:08Z
dc.date.available2015-05-16T02:44:08Z
dc.date.issued1988-04-01en
dc.identifier.citationThe American Journal of Pathology; 131(1): 137-45en
dc.identifier.govdoc3354640en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/12968en
dc.description.abstractReperfusing hearts after an ischemic episode can result in cellular Ca2+ overload. This is accompanied by the formation of contraction bands, loss of sarcolemmal integrity, and mitochondrial disruption. The present study investigated the effect of uncoupling oxidative phosphorylation with 2,4-dinitrophenol (DNP) during reperfusion after 30 or 60 minutes of ischemia on this reperfusion-induced Ca2+ gain. After 60 minutes' ischemia, reperfusion with 1 mM DNP delayed the accumulation of Ca2+ and increased the duration of reperfusion before sarcolemmal disruptions were evident. This suggested that once sarcolemmal integrity is lost, Ca2+ will freely enter the cells irrespective of whether the mitochondria are able to accumulate Ca2+. After 30 minutes ischemia, reperfusion for up to 30 minutes with 0.1 or 1 mM DNP attenuated the Ca2+ gain and maintained sarcolemmal integrity. Because the authors previously found that maintaining sarcolemmal integrity alone does not totally abolish Ca2+ gain, it is suggested that DNP must prevent the entry of Ca2+ that occurs via route(s) other than those created by the loss of sarcolemmal integrity.en
dc.language.isoenen
dc.subject.other2,4-Dinitrophenolen
dc.subject.otherAerobiosisen
dc.subject.otherAnaerobiosisen
dc.subject.otherAnimalsen
dc.subject.otherCalcium.metabolismen
dc.subject.otherCoronary Disease.metabolism.pathologyen
dc.subject.otherDinitrophenols.pharmacologyen
dc.subject.otherFemaleen
dc.subject.otherHeart.drug effectsen
dc.subject.otherIn Vitro Techniquesen
dc.subject.otherKineticsen
dc.subject.otherMicroscopy, Electronen
dc.subject.otherMyocardium.metabolism.ultrastructureen
dc.subject.otherPerfusionen
dc.subject.otherRatsen
dc.subject.otherRats, Inbred Strainsen
dc.titleCalcium gain during postischemic reperfusion. The effect of 2,4-dinitrophenol.en
dc.typeJournal Articleen
dc.identifier.journaltitleThe American journal of pathologyen
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australiaen
dc.description.pages137-45en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/3354640en
dc.type.austinJournal Articleen
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.cerifentitytypePublications-
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