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Title: Calcium gain during postischemic reperfusion. The effect of 2,4-dinitrophenol.
Austin Authors: Elz, J S;Nayler, W G
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 1-Apr-1988
Publication information: The American Journal of Pathology; 131(1): 137-45
Abstract: Reperfusing hearts after an ischemic episode can result in cellular Ca2+ overload. This is accompanied by the formation of contraction bands, loss of sarcolemmal integrity, and mitochondrial disruption. The present study investigated the effect of uncoupling oxidative phosphorylation with 2,4-dinitrophenol (DNP) during reperfusion after 30 or 60 minutes of ischemia on this reperfusion-induced Ca2+ gain. After 60 minutes' ischemia, reperfusion with 1 mM DNP delayed the accumulation of Ca2+ and increased the duration of reperfusion before sarcolemmal disruptions were evident. This suggested that once sarcolemmal integrity is lost, Ca2+ will freely enter the cells irrespective of whether the mitochondria are able to accumulate Ca2+. After 30 minutes ischemia, reperfusion for up to 30 minutes with 0.1 or 1 mM DNP attenuated the Ca2+ gain and maintained sarcolemmal integrity. Because the authors previously found that maintaining sarcolemmal integrity alone does not totally abolish Ca2+ gain, it is suggested that DNP must prevent the entry of Ca2+ that occurs via route(s) other than those created by the loss of sarcolemmal integrity.
Gov't Doc #: 3354640
Journal: The American journal of pathology
Type: Journal Article
Subjects: 2,4-Dinitrophenol
Coronary Disease.metabolism.pathology
Heart.drug effects
In Vitro Techniques
Microscopy, Electron
Rats, Inbred Strains
Appears in Collections:Journal articles

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