Austin Health

Title
Ischaemia causes externalization of endothelin-1 binding sites in rat cardiac membranes.
Publication Date
1989-11-15
Author(s)
Liu, J J
Casley, David J
Nayler, W G
Type of document
Journal Article
Abstract
Specific, high affinity binding sites for iodinated endothelin-1 ([125I]-ET-1) were identified in crude plasma and light membrane fractions harvested from aerobically perfused and ischaemic rat hearts, to determine whether the ischaemia-induced increase in binding site density (Bmax) involves externalization of the sites. In crude plasma membranes Bmax increased after 60 min ischaemia, from 113.5 +/- 2.15 to 180.6 +/- 4.67 fmol/mg protein (p less than 0.01). In the light membranes, the Bmax fell, from 94.7 +/- 8.70 to 63.80 +/- 6.26 fmol/mg protein (p less than 0.05). Hill coefficients and selectivity of both membrane fractions were unchanged. These results are interpreted as meaning that ischaemia causes externalization of cardiac [125I]-ET-1 binding sites.
Link
Citation
Biochemical and Biophysical Research Communications; 164(3): 1220-5
Jornal Title
Biochemical and biophysical research communications

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