Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/12544
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dc.contributor.authorDuggan, K Aen
dc.contributor.authorMendelsohn, Frederick AOen
dc.contributor.authorLevens, N Ren
dc.date.accessioned2015-05-16T02:15:21Z
dc.date.available2015-05-16T02:15:21Z
dc.date.issued1989-10-01en
dc.identifier.citationThe American Journal of Physiology; 257(4 Pt 1): G504-10en
dc.identifier.govdoc2552823en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/12544en
dc.description.abstractThe purpose of this study was to map the distribution of angiotensin II (ANG II) receptors and ANG I-converting enzyme (ACE) in rat intestine. ANG II binding sites were visualized by in vitro autoradiography using iodinated [Sar1, Ile8]ANG II. The distribution of ACE was mapped using an iodinated derivative of lisinopril. Male Sprague-Dawley rats were killed and the interior of the whole intestine washed with ice-cold saline. Segments of duodenum, jejunum, ileum, and colon were quickly frozen in a mixture of isopentane and dry ice. Twenty-micron frozen sections were thaw-mounted onto gelatin-coated slides, incubated with either ligand, and exposed to X-ray film. After exposure and subsequent development, the films were quantitated by computerized densitometry. ANG II receptors were most dense in the colon, followed by the ileum, duodenum, and jejunum. Within each segment of intestine, specific ANG II binding sites were localized exclusively to the muscularis. In contrast, ACE was present in both the mucosa and the muscularis. The colocalization of ANG II receptors and ACE may suggest a role for locally generated ANG II in the control of intestinal function. The luminal orientation of ACE in the mucosa of the small intestine may suggest that at this site ACE serves primarily to hydrolyze dietary peptides.en
dc.language.isoenen
dc.subject.otherAngiotensin II.metabolismen
dc.subject.otherAnimalsen
dc.subject.otherAutoradiographyen
dc.subject.otherBinding, Competitiveen
dc.subject.otherColon.metabolismen
dc.subject.otherDuodenum.metabolismen
dc.subject.otherIleum.metabolismen
dc.subject.otherIntestines.metabolismen
dc.subject.otherIodine Radioisotopesen
dc.subject.otherJejunum.metabolismen
dc.subject.otherKineticsen
dc.subject.otherMaleen
dc.subject.otherMuscle, Smooth.metabolismen
dc.subject.otherPeptidyl-Dipeptidase A.metabolismen
dc.subject.otherRatsen
dc.subject.otherRats, Inbred Strainsen
dc.subject.otherReceptors, Angiotensin.metabolismen
dc.titleAngiotensin receptors and angiotensin I-converting enzyme in rat intestine.en
dc.typeJournal Articleen
dc.identifier.journaltitleAmerican Journal of Physiologyen
dc.identifier.affiliationDepartment of Medicine, Austin Hospital, Melbourne, Victoria, Australiaen
dc.description.pagesG504-10en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/2552823en
dc.type.austinJournal Articleen
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
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