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https://ahro.austin.org.au/austinjspui/handle/1/12250
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DC Field | Value | Language |
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dc.contributor.author | Shulkes, Arthur | en |
dc.contributor.author | Fletcher, D R | en |
dc.contributor.author | Hardy, Kenneth John | en |
dc.date.accessioned | 2015-05-16T01:54:34Z | |
dc.date.available | 2015-05-16T01:54:34Z | |
dc.date.issued | 1989-09-10 | en |
dc.identifier.citation | Journal of Gastroenterology and Hepatology; 4(5): 429-35 | en |
dc.identifier.govdoc | 2491208 | en |
dc.identifier.other | PUBMED | en |
dc.identifier.uri | https://ahro.austin.org.au/austinjspui/handle/1/12250 | en |
dc.description.abstract | Neurotensin (NT), a 13-amino acid peptide, is released from the ileum following a meal. It is metabolized principally by the kidney and in the circulation to N-terminal fragments and apparently rapidly degraded C-terminal fragments. The present study was designed to compare the biological activity (plasma pancreatic polypeptide response) and the clearance kinetics of NT(1-13), the N-terminal fragment NT(1-11) and the C-terminal fragment NT(8-13). To measure accurately the circulating concentrations of short-lived NT fragments in the circulation, a method was devised of collecting blood directly into alcohol ('alcohol fixation'). The alcohol fixation procedure prevented the post-collection losses of the C-terminal fragment NT(8-13) and established that, based on blood levels achieved, NT(8-13) had 70% of the pancreatic polypeptide stimulating potency of NT(1-13). Nevertheless, the metabolic clearance rate of NT(8-13) was about sevenfold higher than the intact molecule, NT(1-13), suggesting that circulating C-terminal fragments have a minor physiological role. When the N-terminal fragment NT(1-11) was infused, there was no sustained effect on pancreatic polypeptide secretion although it was cleared at a rate similar to that of NT(1-13). It is concluded that the use of alcohol fixation prevents post-collection losses of NT fragments and enables true biological potencies of short-lived fragments to be assessed. The biological activity of NT resides in the C-terminus which, once split from the protective N-terminus, is rapidly degraded in the circulation. The remaining intact but inactive N-terminus is relatively stable. | en |
dc.language.iso | en | en |
dc.subject.other | Animals | en |
dc.subject.other | Blood Specimen Collection.methods | en |
dc.subject.other | Ethanol | en |
dc.subject.other | Metabolic Clearance Rate | en |
dc.subject.other | Neurotensin.metabolism | en |
dc.subject.other | Pancreatic Polypeptide.metabolism | en |
dc.subject.other | Peptide Fragments.metabolism | en |
dc.subject.other | Radioimmunoassay | en |
dc.subject.other | Sheep | en |
dc.title | Biological potency of neurotensin metabolites in vivo: importance of alcohol 'fixation' of blood. | en |
dc.type | Journal Article | en |
dc.identifier.journaltitle | Journal of Gastroenterology and Hepatology | en |
dc.identifier.affiliation | Department of Surgery, University of Melbourne, Austin Hospital, Victoria, Australia | en |
dc.description.pages | 429-35 | en |
dc.relation.url | https://pubmed.ncbi.nlm.nih.gov/2491208 | en |
dc.type.austin | Journal Article | en |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.cerifentitytype | Publications | - |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.openairetype | Journal Article | - |
Appears in Collections: | Journal articles |
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