Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/12250
Full metadata record
DC FieldValueLanguage
dc.contributor.authorShulkes, Arthuren
dc.contributor.authorFletcher, D Ren
dc.contributor.authorHardy, Kenneth Johnen
dc.date.accessioned2015-05-16T01:54:34Z
dc.date.available2015-05-16T01:54:34Z
dc.date.issued1989-09-10en
dc.identifier.citationJournal of Gastroenterology and Hepatology; 4(5): 429-35en
dc.identifier.govdoc2491208en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/12250en
dc.description.abstractNeurotensin (NT), a 13-amino acid peptide, is released from the ileum following a meal. It is metabolized principally by the kidney and in the circulation to N-terminal fragments and apparently rapidly degraded C-terminal fragments. The present study was designed to compare the biological activity (plasma pancreatic polypeptide response) and the clearance kinetics of NT(1-13), the N-terminal fragment NT(1-11) and the C-terminal fragment NT(8-13). To measure accurately the circulating concentrations of short-lived NT fragments in the circulation, a method was devised of collecting blood directly into alcohol ('alcohol fixation'). The alcohol fixation procedure prevented the post-collection losses of the C-terminal fragment NT(8-13) and established that, based on blood levels achieved, NT(8-13) had 70% of the pancreatic polypeptide stimulating potency of NT(1-13). Nevertheless, the metabolic clearance rate of NT(8-13) was about sevenfold higher than the intact molecule, NT(1-13), suggesting that circulating C-terminal fragments have a minor physiological role. When the N-terminal fragment NT(1-11) was infused, there was no sustained effect on pancreatic polypeptide secretion although it was cleared at a rate similar to that of NT(1-13). It is concluded that the use of alcohol fixation prevents post-collection losses of NT fragments and enables true biological potencies of short-lived fragments to be assessed. The biological activity of NT resides in the C-terminus which, once split from the protective N-terminus, is rapidly degraded in the circulation. The remaining intact but inactive N-terminus is relatively stable.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherBlood Specimen Collection.methodsen
dc.subject.otherEthanolen
dc.subject.otherMetabolic Clearance Rateen
dc.subject.otherNeurotensin.metabolismen
dc.subject.otherPancreatic Polypeptide.metabolismen
dc.subject.otherPeptide Fragments.metabolismen
dc.subject.otherRadioimmunoassayen
dc.subject.otherSheepen
dc.titleBiological potency of neurotensin metabolites in vivo: importance of alcohol 'fixation' of blood.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of Gastroenterology and Hepatologyen
dc.identifier.affiliationDepartment of Surgery, University of Melbourne, Austin Hospital, Victoria, Australiaen
dc.description.pages429-35en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/2491208en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

10
checked on Mar 28, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.