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Title: Normal phenotype in conditional androgen receptor (AR) exon 3-floxed neomycin-negative male mice.
Austin Authors: Rana, Kesha;Clarke, Michele V;Zajac, Jeffrey D ;Davey, Rachel A;MacLean, Helen E
Affiliation: Department of Medicine, Austin Health, University of Melbourne , Heidelberg, VIC , Australia
Issue Date: 27-Jan-2014
Publication information: Endocrine Research 2014; 39(3): 130-5
Abstract: Androgens (testosterone and dihydrotestosterone) acting via the androgen receptor (AR) are required for male sexual differentiation, and also regulate the development of many other tissues including muscle, fat and bone. We previously generated an AR(lox) mouse line with exon 3 of the AR gene targeted by loxP sites. The deletion of exon 3 is in-frame, so only the DNA binding-dependent actions of the AR are deleted, but non-DNA binding-dependent actions are retained. This line also contained an antibiotic resistance selection cassette, neomycin (neo) in intron 3, which was also flanked by loxP sites. Hemizygous AR(lox) male mice demonstrated a phenotype of hyperandrogenization, with increased mass of androgen-dependent tissues. We hypothesized that this hyperandrogenization was likely to be due to the presence of the neo cassette. In this study, we have generated an AR(lox) neo-negative mouse line, using the EIIa-cre deleter mouse line to remove the neo cassette. Hemizygous AR(lox) neo-negative male mice have a normal phenotype, with normal body mass and normal mass of androgen-dependent tissues including the testis, seminal vesicles, kidney, spleen, heart and retroperitoneal fat. This neo-negative exon 3-targeted mouse line is the only floxed AR mouse line available to study the DNA binding-dependent actions of the AR in a tissue-specific manner, and is suitable for investigation in all tissues. This study demonstrates the importance of removing the selection cassette, which can potentially alter the phenotype of floxed mouse lines even in the absence of detectable effects on target gene expression.
Gov't Doc #: 24467187
DOI: 10.3109/07435800.2013.864303
Type: Journal Article
Subjects: Cre/loxP
gene expression
mouse model
selection cassette
DNA-Binding Proteins.metabolism
Gene Deletion
Gene Knockout Techniques.methods
Mice, Inbred C57BL
Mice, Transgenic
Receptors, Androgen.genetics.metabolism
Appears in Collections:Journal articles

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