Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11965
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dc.contributor.authorGurtler, Volkeren
dc.contributor.authorMayall, Barrie Cen
dc.contributor.authorWang, Jennyen
dc.contributor.authorGhaly-Derias, Shahbanoen
dc.date.accessioned2015-05-16T01:35:59Z
dc.date.available2015-05-16T01:35:59Z
dc.date.issued2013-11-21en
dc.identifier.citationJournal of Virological Methods 2013; 196(): 179-84en
dc.identifier.govdoc24269795en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/11965en
dc.description.abstractIn order to determine the effect of the increase in sensitivity of HCMV detection in whole blood compared to plasma on reproductive rate (Ro) measurement, an optimized human cytomegalovirus (HCMV) quantitative PCR assay was developed. The results presented in this study are summarized by the following three methodological improvements: (i) at values below the limit of quantitation (LOQ) of 60copies/ml, determination of HCMV load was more sensitive with whole blood than plasma, (ii) for the determination of viral load, whole blood was more sensitive than plasma below 1000copies/ml but little difference was observed above 1000copies/ml and (iii) the measurement of "Reproductive Rate" can be affected by imprecise measurement of HCMV viral load in either plasma or whole blood compartments depending on whether samples were taken from patients on antiviral treatment or from patients where HCMV load was rising. Taken together this study provides methodological improvements suggesting that below HCMV viral load levels of 1000copies/ml (1640IU/ml) both plasma and whole blood should be tested.en
dc.language.isoenen
dc.subject.otherHCMVen
dc.subject.otherHuman cytomegalovirusen
dc.subject.otherIUen
dc.subject.otherLODen
dc.subject.otherLOQen
dc.subject.otherPlasmaen
dc.subject.otherQuantitative real-time PCRen
dc.subject.otherR(o)en
dc.subject.otherSNPen
dc.subject.otherWBen
dc.subject.otherWhole blooden
dc.subject.otherhuman cytomegalovirusen
dc.subject.otherinternational unitsen
dc.subject.otherlimit of quantitationen
dc.subject.otherlower limit of detectionen
dc.subject.otherreproductive rateen
dc.subject.othersingle nucleotide polymorphismen
dc.subject.otherwhole blooden
dc.subject.otherBasic Reproduction Numberen
dc.subject.otherBlood.virologyen
dc.subject.otherCytomegalovirus.growth & development.isolation & purificationen
dc.subject.otherCytomegalovirus Infections.epidemiology.virologyen
dc.subject.otherHumansen
dc.subject.otherReal-Time Polymerase Chain Reaction.methodsen
dc.subject.otherSensitivity and Specificityen
dc.subject.otherViral Load.methodsen
dc.titleThe increased sensitivity of human cytomegalovirus (HCMV) PCR quantitation in whole blood affects reproductive rate (Ro) measurement.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of virological methodsen
dc.identifier.affiliationSchool of Applied Sciences, Building 223, Level 1, Bundoora Campus RMIT University, PO Box 71, Bundoora 3083, Australiaen
dc.identifier.affiliationDepartment of Pathology, Austin Hospital, Studley Road, Heidelberg 3084, Australiaen
dc.identifier.doi10.1016/j.jviromet.2013.10.043en
dc.description.pages179-84en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/24269795en
dc.type.austinJournal Articleen
item.grantfulltextnone-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en-
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