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Title: Visualization of mouse barrel cortex using ex-vivo track density imaging.
Austin Authors: Kurniawan, Nyoman D;Richards, Kay L;Yang, Zhengyi;She, David;Ullmann, Jeremy F P;Moldrich, Randal X;Liu, Sha;Yaksic, Javier Urriola;Leanage, Gayeshika;Kharatishvili, Irina;Wimmer, Verena;Calamante, Fernando;Galloway, Graham J;Petrou, Steven;Reutens, David C
Affiliation: Department of Medicine, Austin Health and Northern Health, University of Melbourne, Melbourne, Victoria, Australia
Centre for Advanced Imaging, University of Queensland, Brisbane, Queensland, Australia
Florey Institute of Neuroscience and Mental Health, Melbourne, Victoria, Australia
Queensland Brain Institute, Brisbane, Queensland, Australia
ITEE, University of Queensland, Brisbane, Queensland, Australia
Issue Date: 21-Sep-2013
Publication information: Neuroimage 2013; 87(): 465-75
Abstract: We describe the visualization of the barrel cortex of the primary somatosensory area (S1) of ex vivo adult mouse brain with short-tracks track density imaging (stTDI). stTDI produced much higher definition of barrel structures than conventional fractional anisotropy (FA), directionally-encoded color FA maps, spin-echo T1- and T2-weighted imaging and gradient echo T1/T2*-weighted imaging. 3D high angular resolution diffusion imaging (HARDI) data were acquired at 48 micron isotropic resolution for a (3mm)(3) block of cortex containing the barrel field and reconstructed using stTDI at 10 micron isotropic resolution. HARDI data were also acquired at 100 micron isotropic resolution to image the whole brain and reconstructed using stTDI at 20 micron isotropic resolution. The 10 micron resolution stTDI maps showed exceptionally clear delineation of barrel structures. Individual barrels could also be distinguished in the 20 micron stTDI maps but the septa separating the individual barrels appeared thicker compared to the 10 micron maps, indicating that the ability of stTDI to produce high quality structural delineation is dependent upon acquisition resolution. Close homology was observed between the barrel structure delineated using stTDI and reconstructed histological data from the same samples. stTDI also detects barrel deletions in the posterior medial barrel sub-field in mice with infraorbital nerve cuts. The results demonstrate that stTDI is a novel imaging technique that enables three-dimensional characterization of complex structures such as the barrels in S1 and provides an important complementary non-invasive imaging tool for studying synaptic connectivity, development and plasticity of the sensory system.
Gov't Doc #: 24060319
DOI: 10.1016/j.neuroimage.2013.09.030
Type: Journal Article
Subjects: Barrel cortex
Constrained spherical deconvolution
Diffusion tensor imaging
Diffusion weighted imaging
Diffusion-weighted imaging
Directionally encoded color
Fiber orientation distribution
Fractional anisotropy
High angular-resolution diffusion-weighted imaging
Infraorbital nerve cut
Magnetic resonance
Mouse brain
Posterior medial barrel sub-field
Short-tracks track density imaging
Spherical harmonics
Track density imaging
Track-density imaging
Ventral posteromedial thalamic nucleus
Brain Mapping.methods
Diffusion Magnetic Resonance Imaging.methods
Image Processing, Computer-Assisted.methods
Imaging, Three-Dimensional.methods
Mice, Inbred C57BL
Somatosensory Cortex.anatomy & histology
Appears in Collections:Journal articles

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