Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11874
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dc.contributor.authorKapuscinski, Men
dc.contributor.authorShulkes, Arthuren
dc.contributor.authorRead, D Men
dc.contributor.authorHardy, Kenneth Johnen
dc.date.accessioned2015-05-16T01:30:17Z
dc.date.available2015-05-16T01:30:17Z
dc.date.issued1990-01-01en
dc.identifier.citationThe Journal of Clinical Endocrinology and Metabolism; 70(1): 100-6en
dc.identifier.govdoc2403568en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/11874en
dc.description.abstractEndocrine tumors are useful sources for determining the synthesis and metabolism of secreted regulatory peptides. The present study was performed to compare the synthesis and metabolism of neurotensin (NT) in normal subjects and four patients with NT-producing tumors. NT mRNA was measured and characterized using oligonucleotide probes and Northern blots, while NT-like peptides were quantitated by RIA with region-specific antisera and high pressure liquid chromatography. Northern blot analysis of mRNA isolated from normal human ileum revealed two species of mRNA hybridizing to a heterologous canine oligonucleotide probe; the apparent sizes of the mRNA were 1.4 and 1.0 kilobases. An identical pattern was found in a pancreatic endocrine tumor, a prostatic adenocarcinoma, and a fibrolamellar hepatoma. The ratio of mRNA to peptide varied between the different tissues. For instance, the hepatoma was the richest source of NT mRNA, but the prostatic tumor contained the highest peptide concentration. Measurements with region-specific antisera showed that N-terminal immunoreactive fragments were more abundant than C-terminal fragments in pancreatic, prostatic, and carcinoid tumors (N/C-teminal ratios, 4.0, 1.6, and 5.0) and in equal concentrations in normal ileum. Reverse phase high pressure liquid chromatography revealed the presence of intact NT in addition to a variable number of smaller N-terminal peptides, presumed to be metabolites. In contrast the hepatoma contained a 5-fold excess of C-terminal immunoreactivity. The excess C-terminal immunoreactivity was also present in the circulation of this patient. The chromatographic properties, immunoreactivity, and unusual stability of the C-terminal fragment found in the hepatoma patient suggest that it is a substance distinct from NT itself and is released specifically by the fibrolamellar hepatoma.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherDogsen
dc.subject.otherHumansen
dc.subject.otherIleum.metabolismen
dc.subject.otherLiver Neoplasms.secretionen
dc.subject.otherMaleen
dc.subject.otherMolecular Probe Techniquesen
dc.subject.otherNeurotensin.genetics.secretionen
dc.subject.otherNucleic Acid Hybridizationen
dc.subject.otherPancreatic Neoplasms.secretionen
dc.subject.otherParaneoplastic Endocrine Syndromes.metabolismen
dc.subject.otherProstatic Neoplasms.secretionen
dc.subject.otherRNA, Messenger.isolation & purificationen
dc.subject.otherSheepen
dc.subject.otherSpecies Specificityen
dc.titleExpression of neurotensin in endocrine tumors.en
dc.typeJournal Articleen
dc.identifier.journaltitleThe Journal of Clinical Endocrinology and Metabolismen
dc.identifier.affiliationUniversity of Melbourne, Department of Surgery, Austin Hospital, Australiaen
dc.identifier.doi10.1210/jcem-70-1-100en
dc.description.pages100-6en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/2403568en
dc.type.austinJournal Articleen
item.languageiso639-1en-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.cerifentitytypePublications-
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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