Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11691
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dc.contributor.authorJamieson, Gary Pen
dc.contributor.authorSnook, M Ben
dc.contributor.authorWiley, J Sen
dc.date.accessioned2015-05-16T01:18:33Z
dc.date.available2015-05-16T01:18:33Z
dc.date.issued1990-05-16en
dc.identifier.citationLeukemia Research; 14(5): 475-9en
dc.identifier.govdoc2345470en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/11691en
dc.description.abstractAccumulation of cytosine arabinoside triphosphate (araCTP) from a range of cytosine arabinoside (araC) concentrations (1-50 microM) was measured during incubations of leukemic cells freshly isolated from patients with acute leukemia. In all but one patient, increments in extracellular araC above 10 microM did not increase intracellular araCTP levels. This maximal level of araCTP accumulation ranged from 254 to 1607 pmol/10(7) cells attained after 1 h incubation and did not correlate with either the number of nucleoside transporters on the cell membrane or the Vmax of araC phosphorylation in cell free extracts. Extremely low araCTP accumulation (103 pmol/10(7) cells/h at 50 microM araC) was observed in an AML patient with the unusual finding of micromyeloblasts. These cells also had very low numbers of nucleoside transport sites (less than 500 sites/cell) and were mitotically inactive. The unique feature of the myeloblasts from this patient was that intracellular araCTP accumulation showed a linear dependence on extracellular araC up to 50 microM with no evidence of saturation.en
dc.language.isoenen
dc.subject.otherAcute Diseaseen
dc.subject.otherArabinofuranosylcytosine Triphosphate.pharmacokineticsen
dc.subject.otherArabinonucleotides.pharmacokineticsen
dc.subject.otherBinding Sitesen
dc.subject.otherBiological Transporten
dc.subject.otherCell Membrane.metabolismen
dc.subject.otherCytarabine.administration & dosage.metabolismen
dc.subject.otherDeoxycytidine Kinase.metabolismen
dc.subject.otherExtracellular Space.metabolismen
dc.subject.otherHumansen
dc.subject.otherLeukemia.enzymology.metabolismen
dc.subject.otherLymphocytes.metabolismen
dc.subject.otherNucleosides.metabolismen
dc.subject.otherPhosphorylationen
dc.subject.otherThioinosine.analogs & derivatives.metabolismen
dc.subject.otherTumor Cells, Cultured.enzymology.metabolismen
dc.titleSaturation of intracellular cytosine arabinoside triphosphate accumulation in human leukemic blast cells.en
dc.typeJournal Articleen
dc.identifier.journaltitleLeukemia researchen
dc.identifier.affiliationDepartment of Haematology, Austin Hospital, Heidelberg, Melbourne, Australiaen
dc.description.pages475-9en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/2345470en
dc.type.austinJournal Articleen
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
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