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Title: Lack of cell stress markers in fibrous cap cells in the left main coronary artery.
Austin Authors: Rai, Sudarshan;Zulli, Anthony
Affiliation: Department of Medicine, Austin Health, The University of Melbourne, Heidelberg, Victoria, Australia
Issue Date: 11-Feb-2013
Publication information: Histology and Histopathology 2013; 28(4): 505-11
Abstract: Fibrous cap formation is a key aspect of preventing clinical events but animal models to study this are limited and cellular stress plays a fundamental role in fibrous cap formation.To characterise cellular stress markers in an established animal model to study coronary artery fibrous cap formation.Male New Zealand White rabbits were fed a diet containing 0.5% cholesterol and 1% methionine for 4 weeks, then 9 weeks of normal diet to induce fibrous cap formation. Immunohistochemistry was used to detect CHOP, GRP78, nitrotyrosine HSP70, HSP90, iNOS and HSP32.The core within the left main coronary artery atherosclerosis contained vast amount of foamy macrophages which readily stained for all markers. However, the smooth muscle cells within the formed fibrous cap were negative for all markers. The endothelium overlying the fibrous cap was positive for CHOP, GRP78, nitrotyrosine, iNOS and HSP32, however it was difficult to detect positive endothelial HSP70 or HSP90 immunoreactivity. Serial sectioning and immunohistochemistry for all factors showed clear dual iNOS+ / HSP32 + / HSP70- / HSP90- single cells within the fibrous cap formed.Smooth muscle cells within fibrous caps appear 'stress free', however isolated single smooth muscle cells within caps and within the core show positive immunoreactivity for stress markers. This model could be used to understand the role of cellular stress in fibrous cap formation in the coronary artery.
Gov't Doc #: 23397141
Type: Journal Article
Subjects: Animals
Biological Markers.metabolism
Coronary Artery Disease.metabolism.pathology
Coronary Vessels.metabolism.pathology
Disease Models, Animal
Endothelial Cells.metabolism.pathology
HSP70 Heat-Shock Proteins.metabolism
HSP90 Heat-Shock Proteins.metabolism
Heat-Shock Proteins.metabolism
Heme Oxygenase-1.metabolism
Muscle, Smooth, Vascular.metabolism.pathology
Myocytes, Smooth Muscle.metabolism.pathology
Nitric Oxide Synthase Type II.metabolism
Plaque, Atherosclerotic
Stress, Physiological
Transcription Factor CHOP.metabolism
Tyrosine.analogs & derivatives.metabolism
Appears in Collections:Journal articles

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