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Title: | Ischemia and reperfusion increase 125I-labeled endothelin-1 binding in rat cardiac membranes. | Austin Authors: | Liu, J;Chen, R;Casley, David J;Nayler, W G | Affiliation: | Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia | Issue Date: | 1-Mar-1990 | Publication information: | The American Journal of Physiology; 258(3 Pt 2): H829-35 | Abstract: | The effect of aerobic perfusion, of up to 90 min global ischemia, and of reperfusion on 125I-labeled porcine endothelin (125I-ET-1) binding site density (Bmax), affinity (KD), and selectivity was investigated using cardiac membranes harvested from adult Sprague-Dawley rats. Membranes from nonperfused hearts bound 125I-ET-1 with a Bmax of 117.0 +/- 8.8 fmol/mg protein, a KD of 0.076 +/- 0.005 nM, and a Hill coefficient approaching unity. Neither aerobic perfusion nor 10 min of normothermic ischemia and subsequent reperfusion altered these binding parameters. The extension of the ischemic episode increased Bmax (23, 32, 62, and 60% increase after 20, 30, 60, and 90 min ischemia, respectively). Reperfusion further increased Bmax (P less than 0.01 at 15 min reperfusion after 20 and 30 min ischemia, and after 30 min reperfusion following 60 min ischemia) without changing selectivity. Affinity was reduced after 60 (P less than 0.05) and 90 (P less than 0.01) min ischemia. Ischemia at 22 degrees C had no effect on the density or affinity of these binding sites. | Gov't Doc #: | 2180323 | URI: | https://ahro.austin.org.au/austinjspui/handle/1/11312 | Journal: | American Journal of Physiology | URL: | https://pubmed.ncbi.nlm.nih.gov/2180323 | Type: | Journal Article | Subjects: | Animals Buffers Coronary Disease.metabolism Endothelins Endothelium, Vascular.metabolism Female Hypothermia, Induced In Vitro Techniques Membranes.metabolism Myocardial Reperfusion Myocardium.metabolism Peptides.metabolism Rats Rats, Inbred Strains Temperature |
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