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Title: Ischemia and reperfusion increase 125I-labeled endothelin-1 binding in rat cardiac membranes.
Austin Authors: Liu, J;Chen, R;Casley, David J;Nayler, W G
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia
Issue Date: 1-Mar-1990
Publication information: The American Journal of Physiology; 258(3 Pt 2): H829-35
Abstract: The effect of aerobic perfusion, of up to 90 min global ischemia, and of reperfusion on 125I-labeled porcine endothelin (125I-ET-1) binding site density (Bmax), affinity (KD), and selectivity was investigated using cardiac membranes harvested from adult Sprague-Dawley rats. Membranes from nonperfused hearts bound 125I-ET-1 with a Bmax of 117.0 +/- 8.8 fmol/mg protein, a KD of 0.076 +/- 0.005 nM, and a Hill coefficient approaching unity. Neither aerobic perfusion nor 10 min of normothermic ischemia and subsequent reperfusion altered these binding parameters. The extension of the ischemic episode increased Bmax (23, 32, 62, and 60% increase after 20, 30, 60, and 90 min ischemia, respectively). Reperfusion further increased Bmax (P less than 0.01 at 15 min reperfusion after 20 and 30 min ischemia, and after 30 min reperfusion following 60 min ischemia) without changing selectivity. Affinity was reduced after 60 (P less than 0.05) and 90 (P less than 0.01) min ischemia. Ischemia at 22 degrees C had no effect on the density or affinity of these binding sites.
Gov't Doc #: 2180323
Journal: American Journal of Physiology
Type: Journal Article
Subjects: Animals
Coronary Disease.metabolism
Endothelium, Vascular.metabolism
Hypothermia, Induced
In Vitro Techniques
Myocardial Reperfusion
Rats, Inbred Strains
Appears in Collections:Journal articles

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