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Title: The C-terminal flanking peptide (CTFP) of progastrin inhibits apoptosis via a PI3-kinase-dependent pathway.
Austin Authors: Patel, Oneel;Marshall, Kathryn M;Bramante, Gianni;Baldwin, Graham S;Shulkes, Arthur
Affiliation: Department of Surgery, University of Melbourne Austin Health, Heidelberg, Victoria, Australia 3084, Australia
Issue Date: 19-Aug-2010
Publication information: Regulatory Peptides 2010; 165(2-3): 224-31
Abstract: Progastrin is processed to a number of peptides including glycine-extended gastrin, amidated gastrin and the C-terminal flanking peptide (CTFP). Progastrin and gastrin-gly are pro-proliferative and anti-apoptotic in gastric and colorectal cancer cell lines. The CTFP is a major form of progastrin in the stomach and colon and stimulates proliferation. However the effect of CTFP on apoptosis has not been examined. Using the human gastric carcinoma cell line AGS we show that CTFP attenuates apoptosis through a PI3-kinase pathway by stimulating the phosphorylation of Akt leading to sustained increases in the concentrations of Bcl-xL and phosphorylated Bad protein and by reducing caspase 3 activity. The anti-apoptotic effect represents an important potential mechanism for the growth promoting action of CTFP.
Gov't Doc #: 20727916
DOI: 10.1016/j.regpep.2010.08.005
Journal: Regulatory peptides
Type: Journal Article
Subjects: Apoptosis.drug effects
Blotting, Western
Cell Line, Tumor
Cell Survival.drug effects
Flow Cytometry
Peptide Fragments.chemistry.pharmacology
Phosphatidylinositol 3-Kinases.metabolism
Protein Precursors.chemistry
Signal Transduction.drug effects
Appears in Collections:Journal articles

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