Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/11037
Title: Comparison of histamine-H(1) receptors in bovine intracerebral microvessels with cerebral grey matter by [(3)H]mepyramine binding.
Austin Authors: Culvenor, A J;Jarrott, B
Affiliation: University of Melbourne, Clinical Pharmacology and Therapeutics Unit, Department of Medicine, Austin Hospital, Heidelberg 3084, Victoria, Australia
Issue Date: 16-May-1981
Publication information: Neurochemistry International; 3(6): 365-9
Abstract: Small intracerebral blood vessels (microvessels) of bovine brain are known to contain the vasoactive amine histamine, and the presence of histamine-H(1) receptors in microvessels was examined using the radioligand, [(3)H]mepyramine. Microvessels were isolated from cerebral cortex grey matter, striatum and hippocampus by a sieving technique and membranes prepared for binding studies. [(3)H]Mepyramine bound to a single, high affinity site, which displayed stereoselectivity for (+) chlorpheniramine relative to its (?) isomer and was consistent with binding to H(1)-receptors. The density of binding sites (B(max)), in microvessel membranes from cortical grey matter, was approximately one-third of that seen in membranes prepared from cortical grey matter. Microvessels isolated from striata and hippocampi had a similar density of H(1)-receptor sites to that seen in cortical microvessels. These results demonstrate that bovine intracerebral microvessels contain significant numbers of histamine-H(1) receptors and strengthen the hypothesis that histamine could regulate the calibre of intracerebral blood vessels.
Gov't Doc #: 20487835
URI: https://ahro.austin.org.au/austinjspui/handle/1/11037
Journal: Neurochemistry international
URL: https://pubmed.ncbi.nlm.nih.gov/20487835
Type: Journal Article
Appears in Collections:Journal articles

Show full item record

Page view(s)

14
checked on Jul 19, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.