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Title: Evaluation of a multiplex flow cytometric immunoassay to detect PR3- and MPO-ANCA in active and treated vasculitis, and in inflammatory bowel disease (IBD).
Austin Authors: Trevisin, Michelle;Pollock, Wendy;Dimech, Wayne;Savige, Judy A
Affiliation: Pathology Department, Austin Health, Heidelberg, VIC 3084, Australia
Issue Date: 25-Apr-2008
Publication information: Journal of Immunological Methods 2008; 336(2): 104-12
Abstract: This study compared the performance of a flow cytometric immunoassay for antineutrophil cytoplasmic antibodies (ANCA) directed against proteinase 3 (PR3) and myeloperoxidase (MPO), with indirect immunofluorescence (IIF) and ELISAs from 12 different manufacturers. Sera were from patients with active (n=55) or treated (n=68) small vessel vasculitis, or inflammatory bowel disease (IBD, n=22). The immunoassay specificity was 88% compared with 96% for IIF and 94% (median, range 91-96%) for both ELISAs. Its sensitivity in treated disease was 82% compared with 84% for IIF and 69% (median, range 57-82%) for the ELISAs. The immunoassay's specificity was 88% which was the same as the median for both ELISAs (range 84-95%). The PR3- and MPO-ANCA immunoassay was almost as sensitive as IIF, and more sensitive than, but just as specific as, most ELISAs, in detecting ANCA in active and treated vasculitis. A major advantage of this assay is its ability to be further modified to simultaneously screen for a panel of autoantibodies relevant to vasculitis.
Gov't Doc #: 18499121
DOI: 10.1016/j.jim.2008.03.012
Journal: Journal of immunological methods
Type: Journal Article
Subjects: Antibodies, Antineutrophil Cytoplasmic.blood
Enzyme-Linked Immunosorbent Assay.methods
Flow Cytometry.methods
Fluorescent Antibody Technique, Indirect.methods
Inflammatory Bowel Diseases.blood.diagnosis
Reproducibility of Results
Sensitivity and Specificity
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