Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10572
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dc.contributor.authorZulli, Anthony-
dc.contributor.authorBuxton, Brian F-
dc.contributor.authorBlack, M Jane-
dc.contributor.authorHare, David L-
dc.date.accessioned2015-05-16T00:04:29Z
dc.date.available2015-05-16T00:04:29Z
dc.date.issued2008-06-01en
dc.identifier.citationHistology and Histopathology; 23(6): 741-6en
dc.identifier.govdoc18366012en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/10572en
dc.description.abstractRecent evidence suggests that smooth muscle cells within atherosclerotic plaques originate from vascular progenitor cells. We have previously shown that smooth muscle cells and macrophages present within rabbit atherosclerotic plaques are positive for factors of the renin angiotensin and nitric oxide systems as well as the hematopoietic stem-cell marker CD34 and the pan-leukocyte marker CD45. To explore the idea that these cells are of primitive types, immunohistochemistry was used to identify pluripotent embryonic stem cells (ESC) markers (Oct-4, SSEA1,3,4, TRA1-60, 81) in these plaques and to compare these to intimal thickening.To immunolocalise ESC markers in rabbit aortic intimal thickening and atherosclerotic plaques.New Zealand White rabbits were fed either a control (Con) diet, 0.5% cholesterol (Chol) or 1% methionine (Meth) for 12 weeks. Animals were perfusion fixed, aortae excised and processed for paraffin. Immunohistochemistry was performed by standard techniques.Oct-4, SSEA 1, 3 and 4, TRA-1-60 and TRA-1-81 were all present within in atherosclerotic plaques. However, some cells were not positive for TRA-1-60 and TRA-1-81. In fact, positive TRA-1-81 macrophages were uncommon, and positive TRA-1-81 smooth muscle cells were rare. Intimal thickening in Meth did not show any TRA-1-81 positive cellsMacrophages and smooth muscle cells within atherosclerotic plaques express markers of ESC. These results suggest that cells within these plaques are primitive and might differentiate into other types of cells.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAorta, Thoracic.metabolism.pathologyen
dc.subject.otherAtherosclerosis.metabolism.pathologyen
dc.subject.otherBiological Markers.metabolismen
dc.subject.otherCholesterol, Dietaryen
dc.subject.otherDisease Models, Animalen
dc.subject.otherEmbryonic Stem Cells.metabolism.pathologyen
dc.subject.otherImmunohistochemistryen
dc.subject.otherMacrophages.metabolism.pathologyen
dc.subject.otherMaleen
dc.subject.otherMuscle, Smooth, Vascular.metabolism.pathologyen
dc.subject.otherRabbitsen
dc.subject.otherTunica Intima.metabolism.pathologyen
dc.titleEmbryonic stem cells markers are present within rabbit atherosclerotic plaques.en
dc.typeJournal Articleen
dc.identifier.journaltitleHistology and histopathologyen
dc.identifier.affiliationDepartment of Cardiology, University of Melbourne, Austin Health, Australiaen
dc.description.pages741-6en
dc.identifier.orcid0000-0001-9554-6556-
dc.identifier.pubmedid18366012-
dc.type.austinJournal Articleen
item.fulltextNo Fulltext-
item.openairetypeJournal Article-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
crisitem.author.deptCardiac Surgery-
crisitem.author.deptCardiology-
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