Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10345
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dc.contributor.authorPatel, Oneelen
dc.contributor.authorDumesny, Chelseaen
dc.contributor.authorShulkes, Arthuren
dc.contributor.authorBaldwin, Graham Sen
dc.date.accessioned2015-05-15T23:46:09Z
dc.date.available2015-05-15T23:46:09Z
dc.date.issued2007-03-29en
dc.identifier.citationCancer Letters 2007; 254(1): 87-93en
dc.identifier.govdoc17395367en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/10345en
dc.description.abstractC-terminal fragments from the precursor for gastrin-releasing peptide (GRP) have been detected in several human tumour types. We have previously demonstrated that recombinant human proGRP42-98 is biologically active. To investigate the regions responsible, proGRP42-98 was cleaved with thrombin, and the fragments purified by HPLC. Both proGRP42-79 and proGRP80-98 stimulated proliferation of the human colorectal carcinoma cell line DLD-1, but neither peptide bound to the GRP receptor or bombesin receptor subtype 3. We conclude that two distinct regions of the proGRP C-terminus are biologically active, via a receptor distinct from the known GRP receptors. This discovery opens the way for the development of selective antagonists that may offer new therapies for proGRP-producing tumours.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherBALB 3T3 Cellsen
dc.subject.otherCell Line, Tumoren
dc.subject.otherCell Proliferation.drug effectsen
dc.subject.otherChromatography, High Pressure Liquiden
dc.subject.otherHumansen
dc.subject.otherMiceen
dc.subject.otherPeptide Fragments.genetics.metabolism.pharmacologyen
dc.subject.otherPeptides.chemistry.metabolismen
dc.subject.otherProtein Bindingen
dc.subject.otherProtein Precursors.chemistry.metabolismen
dc.subject.otherRadioligand Assayen
dc.subject.otherReceptors, Bombesin.genetics.metabolismen
dc.subject.otherRecombinant Proteins.isolation & purification.metabolism.pharmacologyen
dc.subject.otherTransfectionen
dc.titleRecombinant C-terminal fragments of the gastrin-releasing peptide precursor are bioactive.en
dc.typeJournal Articleen
dc.identifier.journaltitleCancer lettersen
dc.identifier.affiliationUniversity of Melbourne, Department of Surgery, Austin Health, Heidelberg, Melbourne, Victoria, Australiaen
dc.identifier.doi10.1016/j.canlet.2007.02.014en
dc.description.pages87-93en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/17395367en
dc.type.austinJournal Articleen
item.fulltextNo Fulltext-
item.openairetypeJournal Article-
item.languageiso639-1en-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:Journal articles
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