Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/10252
Title: Poly-L-lysine-coated nanoparticles: a potent delivery system to enhance DNA vaccine efficacy.
Authors: Minigo, Gabriela;Scholzen, Anja;Tang, Choon K;Hanley, Jennifer C;Kalkanidis, Martha;Pietersz, Geoffrey A;Apostolopoulos, Vasso;Plebanski, Magdalena
Affiliation: Vaccine and Infectious Diseases Laboratory, The Burnet Institute incorporating the Austin Research Institute, Austin Hospital, Studley Road, Heidelberg, Victoria 3084, Australia.
Issue Date: 10-Oct-2006
Citation: Vaccine 2006; 25(7): 1316-27
Abstract: DNA formulations provide the basis for safe and cost efficient vaccines. However, naked plasmid DNA is only poorly immunogenic and new effective delivery strategies are needed to enhance the potency of DNA vaccines. In this study, we present a novel approach for the delivery of DNA vaccines using inert poly-L-lysine (PLL) coated polystyrene particles, which greatly enhance DNA immunogenicity. Intradermal injection of plasmid DNA encoding for chicken egg ovalbumin (OVA) complexed with PLL-coated polystyrene nanoparticles induced high levels of CD8 T cells as well as OVA-specific antibodies in C57BL/6 mice and furthermore inhibited tumour growth after challenge with the OVA expressing EG7 tumour cell line. Importantly, vaccine efficacy depended critically on the size of the particles used as well as on the presence of the PLL linker. Our data show that PLL-coated polystyrene nanoparticles of 0.05 microm but not 0.02 microm or 1.0 microm in diameter are highly effective for the delivery of DNA vaccines.
Internal ID Number: 17052812
URI: http://ahro.austin.org.au/austinjspui/handle/1/10252
DOI: 10.1016/j.vaccine.2006.09.086
URL: http://www.ncbi.nlm.nih.gov/pubmed/17052812
Type: Journal Article
Subjects: Adjuvants, Immunologic.pharmacology
Animals
Antibody Formation.drug effects.immunology
CD4-Positive T-Lymphocytes.immunology
CD8-Positive T-Lymphocytes.immunology
Cell Line, Tumor
Chemistry, Pharmaceutical
Dendritic Cells.immunology
Drug Carriers
Drug Delivery Systems
Enzyme-Linked Immunosorbent Assay
Female
Immunity, Cellular.drug effects.immunology
Macrophages, Peritoneal.immunology
Mice
Mice, Inbred BALB C
Nanoparticles
Neoplasm Transplantation
Neoplasms.immunology.prevention & control
Ovalbumin.immunology
Particle Size
Plasmids.genetics.immunology
Polylysine.pharmacology
Polystyrenes
Vaccines, DNA.immunology
Appears in Collections:Journal articles

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