Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10153
Full metadata record
DC FieldValueLanguage
dc.contributor.authorDavey, Rachel Aen
dc.contributor.authorMacLean, Helen Een
dc.date.accessioned2015-05-15T23:30:51Z
dc.date.available2015-05-15T23:30:51Z
dc.date.issued2006-05-09en
dc.identifier.citationAmerican Journal of Physiology. Endocrinology and Metabolism 2006; 291(3): E429-38en
dc.identifier.govdoc16684850en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/10153en
dc.description.abstractGenetically modified mouse models have been used widely to advance our knowledge in the field of endocrinology and metabolism. A number of different approaches to generate genetically modified mice are now available, which provide the power to analyze the role of individual proteins in vivo. However, there are a number of points to be considered in the use and interpretation of these models. This review discusses the advantages and disadvantages involved in the generation and use of different genetically modified mouse models in endocrine research, including conventional techniques (e.g., overexpression, knockout, and knock-in models), tissue- and/or time-specific deletion of target genes [e.g., Cre-loxP and short interfering (si)RNA transgenic approaches], and gene-trap approaches to undertake functional genomics. This review also highlights the many factors that should be considered when assessing the phenotype of these mouse models, many of which are relevant to all murine physiological studies. These approaches are a powerful means by which to dissect the function of genes and are revolutionizing our understanding of endocrine physiology and metabolism.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherEndocrinology.methods.trendsen
dc.subject.otherForecastingen
dc.subject.otherMiceen
dc.subject.otherMice, Knockouten
dc.subject.otherMice, Transgenicen
dc.subject.otherModels, Animalen
dc.subject.otherResearch.trendsen
dc.subject.otherResearch Designen
dc.titleCurrent and future approaches using genetically modified mice in endocrine research.en
dc.typeJournal Articleen
dc.identifier.journaltitleAmerican journal of physiology. Endocrinology and metabolismen
dc.identifier.affiliationDept. of Medicine, University of Melbourne, Austin Health, Heidelberg, VIC 3084, Australiaen
dc.identifier.doi10.1152/ajpendo.00124.2006en
dc.description.pagesE429-38en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/16684850en
dc.type.austinJournal Articleen
item.grantfulltextnone-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

6
checked on Feb 6, 2023

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.