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Title: Current and future approaches using genetically modified mice in endocrine research.
Austin Authors: Davey, Rachel A;MacLean, Helen E
Affiliation: Dept. of Medicine, University of Melbourne, Austin Health, Heidelberg, VIC 3084, Australia
Issue Date: 9-May-2006
Publication information: American Journal of Physiology. Endocrinology and Metabolism 2006; 291(3): E429-38
Abstract: Genetically modified mouse models have been used widely to advance our knowledge in the field of endocrinology and metabolism. A number of different approaches to generate genetically modified mice are now available, which provide the power to analyze the role of individual proteins in vivo. However, there are a number of points to be considered in the use and interpretation of these models. This review discusses the advantages and disadvantages involved in the generation and use of different genetically modified mouse models in endocrine research, including conventional techniques (e.g., overexpression, knockout, and knock-in models), tissue- and/or time-specific deletion of target genes [e.g., Cre-loxP and short interfering (si)RNA transgenic approaches], and gene-trap approaches to undertake functional genomics. This review also highlights the many factors that should be considered when assessing the phenotype of these mouse models, many of which are relevant to all murine physiological studies. These approaches are a powerful means by which to dissect the function of genes and are revolutionizing our understanding of endocrine physiology and metabolism.
Gov't Doc #: 16684850
DOI: 10.1152/ajpendo.00124.2006
Journal: American journal of physiology. Endocrinology and metabolism
Type: Journal Article
Subjects: Animals
Mice, Knockout
Mice, Transgenic
Models, Animal
Research Design
Appears in Collections:Journal articles

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