Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10112
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dc.contributor.authorPang, Ken Cen
dc.contributor.authorWei, Joe Q Zen
dc.contributor.authorChen, Weisanen
dc.date.accessioned2015-05-15T23:27:39Z
dc.date.available2015-05-15T23:27:39Z
dc.date.issued2006-02-20en
dc.identifier.citationJournal of Immunological Methods 2006; 311(1-2): 12-8en
dc.identifier.govdoc16516224en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/10112en
dc.description.abstractIn order to further our basic understanding of antigen processing and presentation as well as to translate that knowledge into clinically effective vaccines and immunotherapies, having appropriate tools to study MHC class I-peptide presentation is highly desirable. Current methods are based upon HPLC fractionation of extracted peptides, monoclonal Ab, multivalent T cell receptors (TCR), T cell hybridomas, TCR transgenic cells, and T cell lines. However, each of these is associated with problems that make them either difficult to apply generally or too insensitive to adequately quantitate antigen presentation. We have developed a method based upon intracellular cytokine staining (ICS) that dynamically and relatively quantitates MHC class I-peptide presentation to CD8+ T cells in a manner that is both widely applicable and highly sensitive. It is well-suited to assess antigen presentation in its early stages, does not require fixation nor labeling of antigen presenting cells (APC), can be used to examine cross-presentation, and is able to directly employ ex vivo T cells which obviates the need for the development and maintenance of T cell lines and hybridomas. Our method represents a simple yet powerful tool that others interested in studying antigen processing and presentation should find of great practical value.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAntigen Presentation.immunologyen
dc.subject.otherBrefeldin A.chemistryen
dc.subject.otherCD8-Positive T-Lymphocytes.immunologyen
dc.subject.otherColoring Agents.chemistryen
dc.subject.otherCytokines.immunology.isolation & purificationen
dc.subject.otherCytotoxicity Tests, Immunologicen
dc.subject.otherDendritic Cells.immunologyen
dc.subject.otherFemaleen
dc.subject.otherFlow Cytometryen
dc.subject.otherHistocompatibility Antigens Class I.immunologyen
dc.subject.otherInfluenza A virus.immunologyen
dc.subject.otherKineticsen
dc.subject.otherMiceen
dc.subject.otherMice, Inbred BALB Cen
dc.subject.otherMice, Inbred C57BLen
dc.subject.otherOvalbumin.immunologyen
dc.subject.otherVaccinia virus.immunologyen
dc.titleDynamic quantification of MHC class I-peptide presentation to CD8+ T cells via intracellular cytokine staining.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of immunological methodsen
dc.identifier.affiliationT cell Laboratory, Ludwig Institute for Cancer Research, Melbourne Branch, Austin Health, Heidelberg, VIC, 3084, Australiaen
dc.identifier.doi10.1016/j.jim.2006.01.008en
dc.description.pages12-8en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/16516224en
dc.type.austinJournal Articleen
item.openairetypeJournal Article-
item.fulltextNo Fulltext-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.cerifentitytypePublications-
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