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Title: Modification of the method of thread manufacture improves stroke induction rate and reduces mortality after thread-occlusion of the middle cerebral artery in young or aged rats.
Austin Authors: Spratt, Neil J;Fernandez, John;Chen, Michelle;Rewell, Sarah;Cox, Susan;van Raay, Leena;Hogan, Lisa;Howells, David William
Affiliation: Department of Medicine, University of Melbourne and National Stroke Research Institute, Austin Health, Heidelberg, Victoria, Australia
Issue Date: 28-Feb-2006
Publication information: Journal of Neuroscience Methods 2006; 155(2): 285-90
Abstract: Improving models of human stroke by the use of aged animals has been advocated; however the commonly used rat middle cerebral artery thread-occlusion model has produced suboptimal stroke induction and excess mortality in aged rats. We report the development of a modified method for silicone-coating the tip of occluding threads which produces a malleable silicone-coated tip which is firmly bonded and of highly consistent diameter, and overcomes problems of thread insertion through the narrowed carotid canal found in aged animals. Comparison of stroke outcomes and mortality were made between these threads and heat-treated poly-L-lysine coated threads. The rate of successful stroke induction in aged rats was significantly improved (from 14% to 86%). Similarly, mortality fell from 21-31% to 3-7% or less in both young and old rats with or without diabetes and hypertension. An occluding thread tip diameter of 0.35-0.38 mm was optimal for induction of mid-sized strokes in both young and old rats. This method of thread manufacture overcomes problems of inconsistency of diameter and bonding of the silicone-coated tip, and these threads produce significant improvements in stroke induction by MCA occlusion, particularly in aged animals and those with co-morbidities.
Gov't Doc #: 16513179
DOI: 10.1016/j.jneumeth.2006.01.020
Type: Journal Article
Subjects: Aging.physiology
Brain Infarction.pathology
Cerebrovascular Circulation.physiology
Constriction, Pathologic.complications
Disease Models, Animal
Infarction, Middle Cerebral Artery.etiology.mortality.pathology.physiopathology
Laser-Doppler Flowmetry.methods
Rats, Inbred SHR
Rats, Inbred WKY
Rats, Sprague-Dawley
Regional Blood Flow.physiology
Silicon Compounds.adverse effects
Tetrazolium Salts.diagnostic use
Appears in Collections:Journal articles

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