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Title: Penetratin tandemly linked to a CTL peptide induces anti-tumour T-cell responses via a cross-presentation pathway.
Austin Authors: Pouniotis, Dodie S;Apostolopoulos, Vasso;Pietersz, Geoffrey A
Affiliation: Immunology and Vaccine Laboratory, The Austin Research Institute, Austin Health, Heidelberg, Victoria, Australia
Issue Date: 1-Mar-2006
Publication information: Immunology; 117(3): 329-39
Abstract: Recently there has been increasing evidence to suggest that membrane translocating peptides enter cells by a receptor-dependent pathway. There have been some studies on the mechanism of major histocompatibility complex (MHC) class I presentation of membrane translocating peptides incorporating cytotoxic T lymphocyte epitopes. However, these have been on different cell lines and only a limited number of inhibitors of the antigen presentation pathway were used. Herein, we demonstrate a comprehensive study utilizing a full spectrum of inhibitors to various pathways of MHC class I to elucidate the mechanism of the membrane translocating peptide, penetratin from Antennapedia (Int). It is clear that Int, RQIKIWFQNRRMKWKK when tandemly linked to a cytotoxic T lymphocyte peptide of ovalbumin, SIINFEKL (IntSIIN) is endocytosed via phagocytosis or macropinocytosis by dendritic cells in an ATP-dependent manner and is processed by a proteasome- and tapasin-independent pathway for presentation and loading to MHC class I molecules. In addition, the majority of antigen is taken up by negatively charged receptors. IntSIIN activates T cells in vitro and in vivo and protects mice against challenge with an ovalbumin-expressing tumour.
Gov't Doc #: 16476052
DOI: 10.1111/j.1365-2567.2005.02304.x
Journal: Immunology
Type: Journal Article
Subjects: Animals
CD8-Positive T-Lymphocytes.immunology
Cancer Vaccines.immunology
Carrier Proteins.immunology
Cells, Cultured
Dendritic Cells.immunology
Epitopes, T-Lymphocyte.immunology
Histocompatibility Antigens Class I.immunology
Lymphocyte Activation.immunology
Mice, Inbred C57BL
Neoplasm Transplantation
Neoplasms, Experimental.immunology.prevention & control
Peptide Fragments.immunology
T-Lymphocytes, Cytotoxic.immunology
Tumor Cells, Cultured
Appears in Collections:Journal articles

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