Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/10088
Full metadata record
DC FieldValueLanguage
dc.contributor.authorMurthi, Padmaen
dc.contributor.authorKalionis, Billen
dc.contributor.authorGhabrial, Hanyen
dc.contributor.authorDunlop, Marjorie Een
dc.contributor.authorSmallwood, R Aen
dc.contributor.authorSewell, Richard Ben
dc.date.accessioned2015-05-15T23:25:49Z
dc.date.available2015-05-15T23:25:49Z
dc.date.issued2006-01-01en
dc.identifier.citationJournal of Gastroenterology and Hepatology; 21(1 Pt 2): 313-8en
dc.identifier.govdoc16460493en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/10088en
dc.description.abstractPrevious studies using isolated perfused rat liver in vivo have suggested that during the erythrocytic phase of malaria infection, overall phagocytosis by Kupffer cells is enhanced. The aim of the present study was to further investigate the individual phagocytic capacity and prostaglandin E(2) (PGE(2)) secretion of isolated Kupffer cells in vitro, and the immunohistochemical characteristics of Kupffer cells in vivo.Malaria was induced in male Sprague-Dawley rats (n = 12) by inoculation with parasitized red cells containing Plasmodium berghei. Kupffer cells were isolated by centrifugal elutriation.A significantly increased yield of Kupffer cells was obtained from malaria-infected livers compared to controls (36.7 +/- 4.5 vs 11.8 +/- 1.1 x10(6) cells, P < 0.0001, n = 12). There was an increased internalization by phagocytosis of [(3)H]-BSA latex microspheres after 60 min in malaria-infected Kupffer cells compared to controls (65.05 +/- 1.5 vs 48.6 +/- 0.7, P < 0.001, n = 12). PGE(2) secretion into the cell culture medium was significantly suppressed in malaria-infected Kupffer cells compared to controls (1167 +/- 88 vs 4537 +/- 383 pg per 10(6) cells, P < 0.001, n = 5). Staining of ED1, ED2 and PCNA was greater in malaria-infected livers compared to control.The results indicate that the number of Kupffer cells is significantly increased and their phagocytic activity on a cell-by-cell basis is enhanced during the erythrocytic stage of malaria.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherCell Counten
dc.subject.otherDinoprostone.secretionen
dc.subject.otherImmunohistochemistryen
dc.subject.otherIn Vitro Techniquesen
dc.subject.otherKupffer Cells.parasitology.physiology.secretionen
dc.subject.otherLiver.metabolism.parasitology.pathologyen
dc.subject.otherMalaria.parasitology.pathology.physiopathologyen
dc.subject.otherMaleen
dc.subject.otherParasitemia.physiopathologyen
dc.subject.otherPhagocytosisen
dc.subject.otherPlasmodium bergheien
dc.subject.otherRatsen
dc.subject.otherRats, Sprague-Dawleyen
dc.titleKupffer cell function during the erythocytic stage of malaria.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of Gastroenterology and Hepatologyen
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Austin and Repatriation Medical Center, Melbourne, Victoria, Australiaen
dc.identifier.doi10.1111/j.1440-1746.2006.04192.xen
dc.description.pages313-8en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/16460493en
dc.type.austinJournal Articleen
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

10
checked on Mar 28, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.