Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9807
Title: A model of partial hepatectomy in mice.
Authors: Nikfarjam, Mehrdad;Malcontenti-Wilson, Caterina;Fanartzis, Michael;Daruwalla, Jurstine;Christophi, Christopher
Affiliation: Department of Surgery, University of Melbourne, Austin Hospital, Melbourne, Victoria, Australia.
Issue Date: 10-Sep-2004
Citation: Journal of Investigative Surgery : the Official Journal of the Academy of Surgical Research; 17(5): 291-4
Abstract: A versatile, simple, and reproducible model of hepatectomy is essential for the study of liver regeneration and its effects on various pathological processes. A murine model of liver resection and regeneration suitable for research is described. Male inbred CBA mice 6-8 wk old were used in all experiments. The contribution of the hepatic lobes to the total liver mass was determined by wet weight measurements. Resection of 37% (n = 10) and 70% (n = 10) liver volume was performed using hemostatic clips to ligate the hepatic lobe pedicles. Animals were recovered and subsequently killed 21 days postoperatively Liver mass was determined and compared to control animals (n = 17) to assess the completeness of liver regeneration. There were no operative deaths in animals undergoing either 37% or 70% hepatectomy. The procedures could be performed expediently, and animal recovery was complete. Liver mass (grams) assessed 21 days postoperatively [mean (SE)] in both the 37% resection, 1.76 g (0.07), and 70% resection, 1.56 g (0.05), groups was not significantly different from control animals, 1.64 g (0.07) (p =.265). Thus, partial hepatectomy can be performed safely and rapidly in mice using haemostatic clip ligation of hepatic lobes, with no impairment to the subsequent process of liver regeneration.
Internal ID Number: 15385262
URI: http://ahro.austin.org.au/austinjspui/handle/1/9807
DOI: 10.1080/08941930490502871
URL: http://www.ncbi.nlm.nih.gov/pubmed/15385262
Type: Journal Article
Subjects: Animals
Hepatectomy.methods
Liver Regeneration
Male
Mice
Mice, Inbred CBA
Appears in Collections:Journal articles

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