Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9798
Title: A robust human T-cell culture method suitable for monitoring CD8+ and CD4+ T-cell responses from cancer clinical trial samples.
Authors: Jackson, Heather M;Dimopoulos, Nektaria;Chen, Qiyuan;Luke, Tina;Yee Tai, Tsin;Maraskovsky, Eugene;Old, Lloyd J;Davis, Ian D;Cebon, Jonathan S;Chen, Weisan
Affiliation: Ludwig Institute for Cancer Research, Melbourne Branch, Austin Health, Heidelberg VIC 3084, Australia.
Issue Date: 1-Aug-2004
Citation: Journal of Immunological Methods; 291(1-2): 51-62
Abstract: Many tumor antigenic determinants have been identified and included in cancer clinical trials. Due to low T-cell frequencies even after vaccination, few T-cell responses can be revealed ex vivo without in vitro stimulation. Various expansion protocols have been employed for this purpose and the outcomes tend to be quite variable, partly due to the high complexity involved in the protocols. Here we systematically studied various common culture conditions including sera, cytokines and feeders and describe a reliable "bulk" culture method that is robust, simpler and more economical. We demonstrated that fetal calf serum (FCS) supported T-cell proliferation better than multiple commercially available pooled human AB sera. IL-2 is critical in our cultures, but IL-7, IL-15 and anti-CTLA-4 in combination with IL-2 did not further enhance T-cell expansion. We typically achieve more than a 40-fold expansion within a 10-day culture period for antigen-specific T cells measured by HLA-peptide tetramer before and after culture. This method was not only validated by multiple operators as a standard operating procedure for monitoring T-cell responses but was also successfully used for discovering novel CD8+ and CD4+ T cells specific to previously unknown epitopes from the NY-ESO-1 tumor antigen.
Internal ID Number: 15345304
URI: http://ahro.austin.org.au/austinjspui/handle/1/9798
DOI: 10.1016/j.jim.2004.04.017
URL: http://www.ncbi.nlm.nih.gov/pubmed/15345304
Type: Journal Article
Subjects: Animals
Antibodies.immunology.pharmacology
Antigen-Presenting Cells.cytology.immunology
Antigens, CD
Antigens, Differentiation.immunology
CD4-Positive T-Lymphocytes.cytology.immunology
CD8-Positive T-Lymphocytes.cytology.immunology
CTLA-4 Antigen
Cattle
Cell Culture Techniques.methods
Cell Division.drug effects
Cells, Cultured
Clinical Trials as Topic
Epitopes.chemistry.immunology
Humans
Interleukin-15.pharmacology
Interleukin-7.pharmacology
Kinetics
Neoplasms.blood.immunology
Peptide Fragments.chemistry.immunology
Reproducibility of Results
Serum
Appears in Collections:Journal articles

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