Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9744
Title: Isolation and characterization of the rat liver AVP receptor using [125I][d(CH2)5'sarcosine7]AVP.
Authors: Trinder, D;Kelly, J M;Fernley, R;Mooser, V;Phillips, P A;Johnston, Colin I
Affiliation: University of Melbourne, Department of Medicine, Austin Hospital, Heidelberg, Australia.
Issue Date: 1-Apr-1992
Citation: Clinical and Experimental Pharmacology & Physiology; 19(4): 253-60
Abstract: 1. A vasopressin (AVP) binding protein was purified from rat liver membranes by an improved method using [125I][d(CH2)5'Sarcosine7]AVP, a selective V1 AVP radioligand and a combination of CHAPS solubilization, gel filtration, lectin affinity and FPLC ion exchange chromatography. 2. The purified protein exhibited a maximum binding activity of 2480 pmol/mg protein with a KD of 4.5 nmol/L, which corresponds to a purification of approximately 26,700-fold. The molecular weight of this protein was 70,000 Da. 3. The binding of [125I][d(CH2)5'Sarcosine7]AVP to the solubilized membranes was dependent on the protein concentration, and was inhibited by the unlabelled peptides [d(CH2)5'Sarcosine7]AVP, AVP, and to a lesser degree by peptides with high V2 receptor affinity, such as 1-desamino-D-AVP and [d(CH2)5'D-Ileu2-Ileu4]AVP. 4. In addition, an AVP anti-idiotypic monoclonal antibody bound to both the partially purified and purified lectin affinity AVP binding protein in a concentration-dependent manner. These results indicate that the purified protein displays similar characteristics to the liver membrane-bound AVP V1 receptor.
Internal ID Number: 1516273
URI: http://ahro.austin.org.au/austinjspui/handle/1/9744
URL: http://www.ncbi.nlm.nih.gov/pubmed/1516273
Type: Journal Article
Subjects: Animals
Antibodies, Monoclonal.diagnostic use
Arginine Vasopressin.analogs & derivatives.diagnostic use.metabolism
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
In Vitro Techniques
Kinetics
Liver.chemistry
Mice
Mice, Inbred BALB C
Molecular Weight
Phospholipids.metabolism
Protein Binding
Rats
Receptors, Angiotensin.drug effects.isolation & purification
Receptors, Vasopressin
Appears in Collections:Journal articles

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