Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9742
Title: Developmental expression and biological activity of gastrin-releasing peptide and its receptors in the kidney.
Authors: Dumesny, Chelsea;Whitley, Jane C;Baldwin, Graham S;Giraud, Andrew S;Shulkes, Arthur
Affiliation: University of Melbourne Department of Surgery, Austin Hospital, Melbourne, Victoria 3084, Australia.
Issue Date: 12-May-2004
Citation: American Journal of Physiology. Renal Physiology 2004; 287(3): F578-85
Abstract: Mammalian gastrin-releasing peptide (GRP) has a widespread distribution and multiple stimulating effects on metabolism, release of regulatory peptides, gastrointestinal and pancreatic secretions, and behavior. GRP is a potent mitogen for a number of tumor types, including colon and lung. Although GRP is known to stimulate the growth of renal tumors, little is known of its synthesis, distribution, and receptors in the developing and mature kidney. Both Northern blot analysis and RT-PCR revealed the presence of GRP mRNA in ovine kidney from midgestation through to adulthood. GRP mRNA was detected in rat kidney from embryonic day 19 to postnatal day 30 by RT-PCR. Sequence-specific radioimmunoassay demonstrated the presence of substantial amounts of fully processed amidated GRP in the ovine renal cortex and medulla. The mRNA for the major receptor subtype, GRP-R, was present in fetal and adult sheep and rat kidneys. The mRNA for the low-affinity GRP receptor, bombesin receptor subtype-3 (BRS-3), was only detected in the rat kidney. In the ovine kidney, immunohistochemistry localized GRP predominantly to the thick ascending limb of the loop of Henle. mRNAs for GRP, GRP-R, and BRS-3 were detected in the human embryonic kidney cell line HEK293, and radioimmunoassay of cell extracts and conditioned media revealed the presence of proGRP but not the amidated form. However, amidated GRP did stimulate the proliferation of these cells. These studies demonstrate that the developing and mature kidney may be previously unidentified sites of autocrine or paracrine action for GRP.
Internal ID Number: 15140764
URI: http://ahro.austin.org.au/austinjspui/handle/1/9742
DOI: 10.1152/ajprenal.00416.2003
URL: http://www.ncbi.nlm.nih.gov/pubmed/15140764
Type: Journal Article
Subjects: Age Factors
Animals
Autocrine Communication.physiology
Blotting, Northern
Cell Division
Cell Line
Fetus
Gastrin-Releasing Peptide.genetics.metabolism
Gene Expression Regulation, Developmental
Humans
Immunohistochemistry
Kidney.cytology.embryology.physiology
Paracrine Communication.physiology
RNA, Messenger.analysis
Rats
Receptors, Bombesin.genetics.metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sheep
Appears in Collections:Journal articles

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