Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9711
Title: Imaging the ischemic penumbra with 18F-fluoromisonidazole in a rat model of ischemic stroke.
Authors: Saita, Kazuko;Chen, Michelle;Spratt, Neil J;Porritt, Michelle J;Liberatore, Gabriel T;Read, Stephen J;Levi, Christopher R;Donnan, Geoffrey A;Ackermann, Uwe;Tochon-Danguy, Henri J;Sachinidis, John I;Howells, David William
Affiliation: Department of Medicine, University of Melbourne, Austin Health, Melbourne, Victoria, Australia.
Issue Date: 11-Mar-2004
Citation: Stroke; A Journal of Cerebral Circulation 2004; 35(4): 975-80
Abstract: The ischemic penumbra is a major focus of stroke research. 18F-fluoromisonidazole (18F-FMISO), a positron emission tomography (PET) marker of hypoxic cells, has shown promise as a technique to image the penumbra in humans. Our aim was to delineate the pattern of 18F-FMISO binding in a rat middle cerebral artery transient thread-occlusion model, and correlate this with tissue outcome at 24 hours. We hypothesized that the pattern of 18F-FMISO binding would mimic that seen in humans.Thirty-eight rats underwent 2 hours transient middle cerebral artery (MCA) occlusion, and then received 18F-FMISO at time points from 0.5 to 22 hours post-MCA occlusion and were killed 2 hours later. Autoradiographic assessment of 18F-FMISO binding and assessment (triphenyltetrazolium chloride) of the area of infarction were performed on tissue slices.Until 1 hour after MCA occlusion, 18F-FMISO binding was increased in the entire MCA territory, with little or no infarction visible. Over the next 5 hours, the pattern of binding evolved to a small rim of intensely binding tissue surrounding the infarct core, which itself showed reduced binding compared with the contralateral hemisphere. By 24 hours, there was minimal accumulation of 18F-FMISO binding and a large area of infarction.The pattern of 18F-FMISO binding rats reproduced the pattern seen in humans, consistent with this tracer being a marker of the ischemic penumbra in both species. This technique may have application in studying the ischemic penumbra in animal models, and correlating this with similar studies in humans.
Internal ID Number: 15017016
URI: http://ahro.austin.org.au/austinjspui/handle/1/9711
DOI: 10.1161/01.STR.0000121647.01941.ba
URL: http://www.ncbi.nlm.nih.gov/pubmed/15017016
Type: Journal Article
Subjects: Animals
Autoradiography
Brain Ischemia.pathology.radionuclide imaging
Infarction, Middle Cerebral Artery.pathology.radionuclide imaging
Male
Misonidazole.analogs & derivatives.analysis
Rats
Rats, Sprague-Dawley
Stroke.pathology.radionuclide imaging
Tomography, Emission-Computed
Appears in Collections:Journal articles

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