Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9635
Full metadata record
DC FieldValueLanguage
dc.contributor.authorLiu, Zhanqien
dc.contributor.authorPanousis, Conen
dc.contributor.authorSmyth, Fiona Een
dc.contributor.authorMurphy, Rogeren
dc.contributor.authorWirth, Veronikaen
dc.contributor.authorCartwright, Glenn Aen
dc.contributor.authorJohns, Terrance Gen
dc.contributor.authorScott, Andrew Men
dc.date.accessioned2015-05-15T22:48:15Z-
dc.date.available2015-05-15T22:48:15Z-
dc.date.issued2003-08-01en
dc.identifier.citationHybridoma and Hybridomics; 22(4): 219-28en
dc.identifier.govdoc14511567en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/9635en
dc.description.abstractThe chimeric monoclonal antibody ch806 specifically targets the tumor-associated mutant epidermal growth factor receptor (de 2-7EGFR or EGFRVIII) and is currently under investigation for its potential use in cancer therapy. The humanised monoclonal antibody hu3S193 specifically targets the Lewis Y epithelial antigen and is currently in Phase I clinical trials in patients with advanced breast, colon, and ovarian carcinomas. To assist the clinical evaluation of ch806 and hu3S193, laboratory assays are required to monitor their serum pharmacokinetics and quantitate any immune responses to the antibodies. Mice immunized with ch806 or hu3S193 were used to generate hybridomas producing antibodies with specific binding to ch806 or hu3S193 and competitive for antigen binding. These anti-idiotype antibodies (designated Ludwig Melbourne Hybridomas, LMH) were investigated as reagents suitable for use as positive controls for HAHA or HACA analyses and for measuring hu3S193 or ch806 in human serum. Anti-idiotypes with the ability to concurrently bind two target antibody molecules were identified, which enabled the development of highly reproducible, sensitive, specific ELISA assays for determining serum concentrations of hu3S193 and ch806 with a 3 ng/mL limit of quantitation using LMH-3 and LMH-12, respectively. BIAcore analyses determined high apparent binding affinity for both idiotypes: LMH-3 binding immobilized hu3S193, Ka = 4.76 x 10(8) M(-1); LMH-12 binding immobilised ch806, Ka = 1.74 x 10(9) M(-1). Establishment of HAHA or HACA analysis of sera samples using BIAcore was possible using LMH-3 and LMH-12 as positive controls for quantitation of immune responses to hu3S193 or ch806 in patient sera. These anti-idiotypes could also be used to study the penetrance and binding of ch806 or hu3S193 to tumor cells through immunohistochemical analysis of tumor biopsies. The generation of anti-idiotype antibodies capable of concurrently binding a target antibody on each variable domain provides reagents with high sensitivity for the assessment of safety and pharmacokinetic profiles of target antibodies administered clinically.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherAntibodies, Anti-Idiotypic.biosynthesis.therapeutic useen
dc.subject.otherAntibody Affinityen
dc.subject.otherCell Line, Tumoren
dc.subject.otherClone Cells.immunologyen
dc.subject.otherCloning, Molecularen
dc.subject.otherEnzyme-Linked Immunosorbent Assayen
dc.subject.otherFemaleen
dc.subject.otherHumansen
dc.subject.otherHybridomas.immunologyen
dc.subject.otherImmunotherapyen
dc.subject.otherLaboratoriesen
dc.subject.otherLewis Blood-Group System.immunologyen
dc.subject.otherMiceen
dc.subject.otherMice, Inbred BALB Cen
dc.subject.otherPlasmacytoma.immunology.pathologyen
dc.subject.otherRecombinant Fusion Proteins.metabolism.pharmacokineticsen
dc.subject.otherReproducibility of Resultsen
dc.subject.otherSerum.immunologyen
dc.subject.otherSurface Plasmon Resonanceen
dc.titleGeneration of anti-idiotype antibodies for application in clinical immunotherapy laboratory analyses.en
dc.typeJournal Articleen
dc.identifier.journaltitleHybridoma and hybridomicsen
dc.identifier.affiliationTumor Targeting Laboratory, Ludwig Institute for Cancer Research, Melbourne Tumor Biology Branch, Austin & Repatriation Medical Centre, 145-164 Studley Road, Heidelberg, Victoria 3084, Australiaen
dc.identifier.doi10.1089/153685903322328947en
dc.description.pages219-28en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/14511567en
dc.type.austinJournal Articleen
local.name.researcherScott, Andrew M
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
crisitem.author.deptMolecular Imaging and Therapy-
crisitem.author.deptOlivia Newton-John Cancer Research Institute-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

18
checked on Mar 27, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.