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dc.contributor.authorGumley, T Pen
dc.contributor.authorMcKenzie, Ian F Cen
dc.contributor.authorKozak, C Aen
dc.contributor.authorSandrin, Mauro Sen
dc.identifier.citationJournal of Immunology (baltimore, Md. : 1950); 149(8): 2615-8en
dc.description.abstractThe Thb locus is responsible for the expression of 15-kDa phosphatidyl inositol anchored molecules (ThB) on murine thymocytes and B cells. Thb expression as detected with mAb is polymorphic on B cells with two alleles, Thbh and Thb1 responsible for the high and low expression of ThB on B cells. The regulatory locus for Thb expression had been mapped with the Ly-6 cluster of genes to Chr 15. In our study we used expression cloning in COS cells to isolate cDNA clones that code for ThB after transfection; the cDNA products react with anti- ThB antibodies, but not with Ly-6A.2, -6B.2, -6C.2, or -6D.2 antibodies. One of these clones, pThB-A contains insert of 702 bases which was sequenced. The translated amino acid sequence has 11 cysteine residues, and together with the absence of potential N-linked glycosylation sites is similar to the structure of the Ly-6 molecules. The nucleotide and amino acid sequences of ThB cDNA were compared to those of Ly-6 genes and the Ly-6 related human CD59 and show clear homology. Finally using interspecies crosses, the structural Thb gene has been mapped to Chr 15; thus both structural and regulatory genes map to a similar site. The genetic map location near Ly-6 and the sequence similarity suggest that Thb and Ly-6 may have been derived from the same progenitor by gene duplication.en
dc.subject.otherAmino Acid Sequenceen
dc.subject.otherAntigens, Ly.geneticsen
dc.subject.otherAntigens, Surface.geneticsen
dc.subject.otherBase Sequenceen
dc.subject.otherBlotting, Northernen
dc.subject.otherBlotting, Southernen
dc.subject.otherChromosome Mappingen
dc.subject.otherDNA.chemistry.isolation & purificationen
dc.subject.otherMolecular Sequence Dataen
dc.titleIsolation and characterization of cDNA clones for the mouse thymocyte B cell antigen (ThB).en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of Immunology (Baltimore, Md. : 1950)en
dc.identifier.affiliationAustin Research Institute, Austin Hospital, Heidelberg, Victoria, Australiaen
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