Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9574
Title: Achlorhydria induced changes in gastrin, somatostatin, H+/K(+)-ATPase and carbonic anhydrase in the sheep.
Authors: Read, M A;Read, D M;Kapuscinski, M;Shulkes, Arthur
Affiliation: Department of Surgery, University of Melbourne, Austin Hospital, Australia.
Issue Date: 2-Jul-1992
Citation: Regulatory Peptides; 40(1): 13-27
Abstract: Gastrin, somatostatin, H+/K(+)-ATPase and carbonic anhydrase are principal elements of acid secretion. We investigated in the conscious sheep the effect of 24 h omeprazole (an H+/K(+)-ATPase inhibitor) infusion on these elements at the level of synthesis, storage and secretion. Omeprazole inhibited acid secretion-pH increased from 3.0 to 7.1 at 24 h. Plasma amidated and glycine extended gastrin increased 3-fold while the ratio of amidated to glycine extended gastrins (4:1) remained unchanged. Despite the increase in circulating gastrin, antral gastrin concentration and mRNA did not change significantly. Gastrin-17 (amidated and glycine extended) was the predominant form in the circulation and antrum, although there were preferential increases in larger forms following omeprazole treatment. Omeprazole had no effect on somatostatin mRNA or peptide levels in the fundus. Similarly, plasma somatostatin remained unchanged. However, antral somatostatin increased significantly (63%) following omeprazole treatment accompanied by a 4-fold increase in its mRNA. Fundic H+/K(+)-ATPase mRNA was unchanged but a significant increase (87%) in carbonic anhydrase II mRNA was observed. Omeprazole induced hypergastrinaemia occurred without a measurable reduction in storage or increased synthesis of gastrin at 24 h. Increased antral somatostatin synthesis and storage may result from stimulation by plasma gastrin on antral D cells, independent of acid. The rise in carbonic anhydrase II mRNA in the absence of any change in H+/K(+)-ATPase mRNA may reflect the differential sensitivity of the genes encoding these two enzymes to the stimulatory action of gastrin.
Internal ID Number: 1359610
URI: http://ahro.austin.org.au/austinjspui/handle/1/9574
URL: http://www.ncbi.nlm.nih.gov/pubmed/1359610
Type: Journal Article
Subjects: Achlorhydria.chemically induced.metabolism
Animals
Base Sequence
Blotting, Northern
Carbonic Anhydrases.metabolism
Chromatography, Gel
Chromatography, High Pressure Liquid
Female
Gastrins.blood.metabolism
H(+)-K(+)-Exchanging ATPase.metabolism
Kinetics
Molecular Sequence Data
Omeprazole
RNA, Messenger.isolation & purification
Radioimmunoassay
Sheep
Somatostatin.metabolism
Appears in Collections:Journal articles

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