Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9549
Title: Characterization of angiotensin converting enzyme in isolated cerebral microvessels from spontaneously hypertensive and normotensive rats.
Authors: Perich, R;Jackson, B;Paxton, D;Johnston, Colin I
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Victoria, Australia.
Issue Date: 1-Feb-1992
Citation: Journal of Hypertension; 10(2): 149-53
Abstract: Abnormalities in the vascular renin-angiotensin system have been hypothesized to contribute to the pathogenesis and complications of hypertension. In animal models of hypertension, there is wide variation in reported vascular angiotensin converting activity, particularly in cerebral microvessels. In this study, we sought to characterize, quantitate and compare cerebral microvessel angiotensin converting enzyme (ACE) in genetically hypertensive rats and normotensive rats.Brain microvascular ACE from 14-week-old spontaneously hypertensive rats (SHR) was measured and compared with ACE from brain microvessels of normotensive Wistar-Kyoto (WKY) controls.Isolated cerebral microvascular ACE was measured using two methods, enzyme kinetic assay or radioligand binding assay.In SHR, cerebral microvessel ACE was of similar activity and concentration and had similar ligand binding affinities to WKY rats. Plasma ACE activity was significantly elevated in WKY rats compared with SHR.Cerebral microvascular ACE is similar in SHR and WKY rats. Microvascular ACE is unlikely to participate in the pathogenesis or complications of hypertension in this model.
Internal ID Number: 1313477
URI: http://ahro.austin.org.au/austinjspui/handle/1/9549
URL: http://www.ncbi.nlm.nih.gov/pubmed/1313477
Type: Journal Article
Subjects: Animals
Brain.blood supply.enzymology
Hypertension.enzymology.genetics
Male
Microcirculation.enzymology
Peptidyl-Dipeptidase A.metabolism
Radioligand Assay
Rats
Rats, Inbred SHR
Rats, Inbred WKY
Appears in Collections:Journal articles

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