Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9510
Title: In vitro autoradiography reveals predominantly AT1 angiotensin II receptors in rat kidney.
Authors: Zhuo, J;Song, K;Harris, P J;Mendelsohn, Frederick AO
Affiliation: Department of Medicine, University of Melbourne, Austin Hospital, Heidelberg, Vic., Australia.
Issue Date: 10-Sep-1992
Citation: Renal Physiology and Biochemistry; 15(5): 231-9
Abstract: Angiotensin II (Ang II) receptor subtypes in the rat kidney were investigated by using type 1 (AT1) and type 2 (AT2) Ang II receptor antagonists to discriminate specific 125I-[Sar1,Ile8] Ang II binding sites with in vitro autoradiography. DuP 753, a nonpeptide Ang II antagonist specific for the AT1 sites, potently displaced binding in glomeruli (Ki = 23.9 +/- 3.3 nM) and proximal tubules (Ki = 43.4 +/- 17 nM). By contrast, the AT2 antagonists, PD 123177 and CGP 42112A, were very weak in competing for specific 125I-[Sar1,Ile8] Ang II binding sites. AT1 receptors, as determined in the presence of an excess concentration (10 microM) of the AT2 antagonist, PD 123177, account for 95% of total renal Ang II receptors, whereas AT2 receptors, as determined in the presence of an excess concentration (10 microM) of the AT1 antagonist, DuP 753, represent approximately 5% of total renal Ang II receptors. In addition, the reducing agent, dithiothreitol, produces a dose-dependent inhibition of Ang II receptor binding with an IC50 of 2 mM, a characteristic of the AT1 receptors. These findings indicate that the AT1 receptor is the predominant subtype at multiple anatomical sites in the rat kidney.
Internal ID Number: 1279763
URI: http://ahro.austin.org.au/austinjspui/handle/1/9510
URL: http://www.ncbi.nlm.nih.gov/pubmed/1279763
Type: Journal Article
Subjects: Angiotensin Receptor Antagonists
Animals
Autoradiography
Binding, Competitive
Kidney.chemistry.radiography
Male
Rats
Rats, Sprague-Dawley
Receptors, Angiotensin.analysis.drug effects
Tissue Distribution
Appears in Collections:Journal articles

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