Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9476
Title: Calcitonin receptor isoforms expressed in the developing rat kidney.
Authors: Tikellis, Christos;Xuereb, Loredanna;Casley, David J;Brasier, Geoffrey;Cooper, Mark E;Wookey, Peter J
Affiliation: Department of Medicine, University of Melbourne, Austin and Repatriation Medical Centre, Repatriation Campus, Heidelberg West, Victoria, Australia.
Issue Date: 1-Feb-2003
Citation: Kidney International; 63(2): 416-26
Abstract: Development in the metanephric-kidney transition period involves the precise expression of paracrine and autocrine events in an ordered spatio-temporal manner. Expression of these molecular events is tightly controlled and includes positive and negative growth factors and cognate receptors within close proximity in developing structures in the expanding renal cortex and medulla. The expression of calcitonin receptor (CTR) isoforms C1a and C1b in this context has not previously been described. Our current study also explored the relationship between the expression of CTR isoforms and amylin binding sites.Techniques included immunohistochemistry with novel antibodies that detect CTR isoforms, real time PCR for the quantification of CTR isoforms, Western blot and in vitro autoradiography, on tissues from embryo day 18 to postnatal day 30.The CTR C1a isoform is expressed in the ureteric ducts of the metanephros and both isoforms are expressed in the developing distal convoluted tubules, ascending limbs of the loop of Henle and collecting ducts in the postnatal rat kidney. There was a 60-fold excess of C1a versus C1b isoforms. An apparent molecular weight of 63 kD was found. In vitro autoradiography demonstrated that while amylin binding sites were predominantly in the cortex, CTR expression was largely localized in the medulla in an earlier event, followed by cortical expression.CTR C1a protein expression has been identified in the ureteric ducts in the metanephros and both isoforms expressed in the distal portions of the developing nephrons and collecting ducts. Since amylin binding sites have been localized on the proximal tubules of the cortex, it is unlikely that amylin receptors can be represented by modification of CTR affinity with receptor activity modifying proteins in the kidney.
Internal ID Number: 12631107
URI: http://ahro.austin.org.au/austinjspui/handle/1/9476
DOI: 10.1046/j.1523-1755.2003.00754.x
URL: http://www.ncbi.nlm.nih.gov/pubmed/12631107
Type: Journal Article
Subjects: Aging.metabolism
Amyloid.metabolism
Animals
Animals, Newborn.growth & development.metabolism
Autoradiography
Binding Sites
Embryo, Mammalian.physiology
Embryonic and Fetal Development
Immunohistochemistry
Islet Amyloid Polypeptide
Kidney.embryology.growth & development.metabolism
Kidney Cortex.metabolism
Kidney Medulla.metabolism
Molecular Weight
Protein Isoforms.metabolism
Rats
Rats, Sprague-Dawley
Receptors, Calcitonin.chemistry.metabolism
Tissue Distribution
Appears in Collections:Journal articles

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