Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9443
Full metadata record
DC FieldValueLanguage
dc.contributor.authorBach, Leon Aen
dc.contributor.authorLeeding, K Sen
dc.date.accessioned2015-05-15T22:32:30Z
dc.date.available2015-05-15T22:32:30Z
dc.date.issued2002-09-01en
dc.identifier.citationHormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Me´tabolisme; 34(9): 487-91en
dc.identifier.govdoc12412602en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/9443en
dc.description.abstractInsulin-like growth factors (IGFs) stimulate proliferation and differentiation of PC12 rat pheochromocytoma cells and modulate catecholamine release in bovine adrenal medullary cells. Dexamethasone increases catecholamine synthesis in PC12 cells. We therefore studied the effects of IGFs and dexamethasone on catecholamine content in PC12 cells. Dopamine (DA) and norepinephrine (NE) content of PC12 cells were measured after incubation for 72 h with IGFs (100 ng/ml) and/or dexamethasone (500 nM). IGF-I (100 ng/ml) and IGF-II (100 ng/ml) decreased DA and NE content to approximately 35% and approximately 25% of control, respectively. [Leu27]IGF-II, which binds to the IGF-I receptor with markedly decreased affinity, did not reduce catecholamine levels, indicating that the effect is likely to be mediated by the IGF-I receptor. Dexamethasone (500 nM) increased levels of DA and NE to 173 +/- 20% and 331 +/- 48% of controls, respectively. Coincubation with IGFs did not significantly affect the stimulation of DA by dexamethasone, but abolished the rise in NE. Levels of tyrosine hydroxylase mRNA, protein and activity were increased following incubation with dexamethasone, but were unchanged by IGFs. These results indicate that IGFs decrease catecholamine content in PC12 cells via the IGF-I receptor. Complex regulation involving multiple synthetic and/or degradative steps is implicated in this process.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherDexamethasone.pharmacologyen
dc.subject.otherDopamine.biosynthesis.metabolismen
dc.subject.otherDrug Interactionsen
dc.subject.otherGlucocorticoids.pharmacologyen
dc.subject.otherInsulin-Like Growth Factor I.pharmacologyen
dc.subject.otherInsulin-Like Growth Factor II.pharmacologyen
dc.subject.otherNorepinephrine.biosynthesis.metabolismen
dc.subject.otherPC12 Cellsen
dc.subject.otherRatsen
dc.subject.otherReceptor, IGF Type 1.metabolismen
dc.subject.otherTyrosine 3-Monooxygenase.metabolismen
dc.titleInsulin-like growth factors decrease catecholamine content in PC12 rat pheochromocytoma cells.en
dc.typeJournal Articleen
dc.identifier.journaltitleHormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolismeen
dc.identifier.affiliationUniversity of Melbourne, Department of Medicine, Austin & Repatriation Medical Centre, Heidelberg, Victoria, Australiaen
dc.identifier.doi10.1055/s-2002-35700en
dc.description.pages487-91en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/12412602en
dc.type.austinJournal Articleen
item.openairetypeJournal Article-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.grantfulltextnone-
item.fulltextNo Fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
Appears in Collections:Journal articles
Show simple item record

Page view(s)

4
checked on Mar 27, 2024

Google ScholarTM

Check


Items in AHRO are protected by copyright, with all rights reserved, unless otherwise indicated.