Please use this identifier to cite or link to this item: https://ahro.austin.org.au/austinjspui/handle/1/9401
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dc.contributor.authorForbes, Josephine Men
dc.contributor.authorBonnet, Fabriceen
dc.contributor.authorRusso, Leileata Men
dc.contributor.authorBurns, Wendy Cen
dc.contributor.authorCao, Zeminen
dc.contributor.authorCandido, Riccardoen
dc.contributor.authorKawachi, Hiroshien
dc.contributor.authorAllen, Terri Jen
dc.contributor.authorCooper, Mark Een
dc.contributor.authorJerums, Georgeen
dc.contributor.authorOsicka, Tanya Men
dc.date.accessioned2015-05-15T22:28:59Z
dc.date.available2015-05-15T22:28:59Z
dc.date.issued2002-05-01en
dc.identifier.citationJournal of Hypertension; 20(5): 985-92en
dc.identifier.govdoc12011660en
dc.identifier.otherPUBMEDen
dc.identifier.urihttps://ahro.austin.org.au/austinjspui/handle/1/9401en
dc.description.abstractNephrin, a cytoskeletal protein which localizes to the slit pore of podocytes, may play a role in proteinuria. This study examines the possible relationship between nephrin expression and albuminuria in normotensive and hypertensive diabetic rats.Streptozotocin diabetes was induced in both Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats. Diabetic and control animals were sacrificed and the kidneys obtained after 8, 16 and 24 weeks. The glomerular filtration rate (GFR) and albuminuria were also measured. Glycaemic control was assessed by measurement of plasma glucose and glycated haemoglobin (HbA1c). Nephrin gene expression was quantitated by real-time polymerase chain reaction (PCR) and localized by in situ hybridization. Nephrin protein expression was localized by immunohistochemistry and quantitated.Following a transient rise at 8 weeks in the diabetic SHR (P < 0.05 versus control SHRs), nephrin gene expression, as determined by real-time PCR, was significantly decreased at 16 and 24 weeks (P < 0.05 versus control SHRs). In situ hybridization confirmed similar changes in nephrin gene expression, which were confined to the glomeruli. This reduction in glomerular nephrin gene expression was associated with increasing albuminuria at 16 and 24 weeks in diabetic SHRs. There were no significant changes in nephrin gene expression, either by real-time reverse transcription polymerase chain reaction or in situ hybridization, observed in normotensive diabetic WKY rats, in the context of much less albuminuria in this group. Immunohistochemistry for nephrin protein revealed a greater depletion in renal nephrin content in SHR than in WKY rats after 24 weeks of diabetes.Reduction in renal nephrin gene and protein expression is closely associated with the development of albuminuria, as observed in an experimental model of diabetes and hypertension.en
dc.language.isoenen
dc.subject.otherAlbuminuria.etiologyen
dc.subject.otherAnimalsen
dc.subject.otherDiabetes Mellitus, Experimental.metabolismen
dc.subject.otherDiabetic Angiopathies.complications.metabolism.urineen
dc.subject.otherHypertension.metabolism.urineen
dc.subject.otherKidney.metabolismen
dc.subject.otherMaleen
dc.subject.otherMembrane Proteinsen
dc.subject.otherProteins.genetics.metabolismen
dc.subject.otherRNA, Messenger.metabolismen
dc.subject.otherRatsen
dc.subject.otherRats, Inbred SHRen
dc.subject.otherRats, Inbred WKYen
dc.subject.otherTissue Distributionen
dc.titleModulation of nephrin in the diabetic kidney: association with systemic hypertension and increasing albuminuria.en
dc.typeJournal Articleen
dc.identifier.journaltitleJournal of Hypertensionen
dc.identifier.affiliationDepartment of Medicine and Endocrinology, University of Melbourne, Austin, Australiaen
dc.description.pages985-92en
dc.relation.urlhttps://pubmed.ncbi.nlm.nih.gov/12011660en
dc.type.austinJournal Articleen
local.name.researcherJerums, George
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextNo Fulltext-
item.grantfulltextnone-
item.languageiso639-1en-
item.openairetypeJournal Article-
crisitem.author.deptEndocrinology-
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