Please use this identifier to cite or link to this item: http://ahro.austin.org.au/austinjspui/handle/1/9264
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dc.contributor.authorBatchelor, Peter Egertonen
dc.contributor.authorLiberatore, G Ten
dc.contributor.authorPorritt, Michelle Jen
dc.contributor.authorDonnan, Geoffrey Aen
dc.contributor.authorHowells, David Williamen
dc.date.accessioned2015-05-15T22:17:24Z
dc.date.available2015-05-15T22:17:24Z
dc.date.issued2000-10-01en
dc.identifier.citationThe European Journal of Neuroscience; 12(10): 3462-8en
dc.identifier.govdoc11029615en
dc.identifier.otherPUBMEDen
dc.identifier.urihttp://ahro.austin.org.au/austinjspui/handle/1/9264en
dc.description.abstractAfter striatal injury, sprouting dopaminergic fibres grow towards and intimately surround wound macrophages which, together with microglia, express the dopaminergic neurotrophic factors glial cell line-derived neurotrophic factor (GDNF) and brain derived neurotrophic factor (BDNF). To evaluate the importance of these endogenously secreted neurotrophic factors in generating striatal peri-wound dopaminergic sprouting, the peri-wound expression of BDNF or GDNF was inhibited by intrastriatal infusion of antisense oligonucleotides for 2 weeks in mice. Knock-down of both BDNF and GDNF mRNA and protein levels in the wounded striatum were confirmed by in situ hybridization and enzyme-linked immunosorbent assay, respectively. Dopamine transporter immunohisto-chemistry revealed that inhibition of either BDNF or GDNF expression resulted in a marked decrease in the intensity of peri-wound sprouting. Quantification of this effect using [H3]-mazindol autoradiography confirmed that peri-wound sprouting was significantly reduced in mice receiving BDNF or GDNF antisense infusions whilst control infusions of buffered saline or sense oligonucleotides resulted in the pronounced peri-wound sprouting response normally associated with striatal injury. BDNF and GDNF thus appear to be important neurotrophic factors inducing dopaminergic sprouting after striatal injury.en
dc.language.isoenen
dc.subject.otherAnimalsen
dc.subject.otherBrain Injuries.drug therapy.pathology.physiopathologyen
dc.subject.otherBrain-Derived Neurotrophic Factor.drug effects.genetics.metabolismen
dc.subject.otherDopamine.metabolismen
dc.subject.otherGlial Cell Line-Derived Neurotrophic Factoren
dc.subject.otherGrowth Cones.drug effects.metabolismen
dc.subject.otherMaleen
dc.subject.otherMiceen
dc.subject.otherMice, Inbred C57BLen
dc.subject.otherNeostriatum.metabolism.physiopathology.surgeryen
dc.subject.otherNerve Growth Factorsen
dc.subject.otherNerve Regeneration.drug effects.geneticsen
dc.subject.otherNerve Tissue Proteins.drug effects.genetics.metabolismen
dc.subject.otherNeuronal Plasticity.drug effects.geneticsen
dc.subject.otherOligonucleotides, Antisense.pharmacologyen
dc.subject.otherRNA, Messenger.metabolismen
dc.subject.otherWound Healing.drug effects.geneticsen
dc.titleInhibition of brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression reduces dopaminergic sprouting in the injured striatum.en
dc.typeJournal Articleen
dc.identifier.journaltitleThe European journal of neuroscienceen
dc.identifier.affiliationDepartment of Medicine, University of Melbourne, Austin, Australia.en
dc.description.pages3462-8en
dc.relation.urlhttp://www.ncbi.nlm.nih.gov/pubmed/11029615en
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